Manipulation of renal gene expression using oligonucleotides

Ronak Lakhia, Abheepsa Mishra, Vishal Patel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Oligonucleotides are small molecules 8–50 nucleotides in length that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA. The use of oligonucleotides to manipulate gene expression in the kidney could be a valuable tool to further understand kidney pathophysiology and can serve as an important complement to genetic studies. This chapter serves as a primer on the use of oligonucleotides in the kidney. We provide an overview of the various ways that oligonucleotides can manipulate gene expression. In addition, we describe the advancements in the development of oligonucleotides for laboratory and clinical use. Finally, instruction is provided on the design and implementation of oligonucleotides for in vitro and in vivo laboratory studies.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
EditorsThomas Weimbs
PublisherAcademic Press Inc.
Pages109-120
Number of pages12
ISBN (Print)9780128203354
DOIs
StatePublished - 2019

Publication series

NameMethods in Cell Biology
Volume154
ISSN (Print)0091-679X

Keywords

  • Antisense oligonucleotides
  • Gene expression
  • MicroRNA
  • Oligonucleotides
  • RNA
  • Therapeutics

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Lakhia, R., Mishra, A., & Patel, V. (2019). Manipulation of renal gene expression using oligonucleotides. In T. Weimbs (Ed.), Methods in Cell Biology (pp. 109-120). (Methods in Cell Biology; Vol. 154). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.05.006