Mannose 6-phosphate receptor (MPR 300) proteins from goat and chicken bind human IGF-II

Suresh Koduru, Sivaramakrishna Yadavalli, Siva Kumar Nadimpalli

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Mannose 6-phosphate receptor proteins (MPR 300 and 46) in mammals have been shown to mediate transport of lysosomal enzymes to lysosomes intracellularly. Both receptors are also expressed on the plasma membrane. Only MPR 300 protein on the plasma membrane has been shown to be a multifunctional protein which in addition to binding mannose 6-phosphate containing proteins also binds human insulin-like growth factor-II (IGF-II) causing its internalization [Hille-Rehfeld, A. (1995) Mannose 6-phosphate receptors in sorting and transport of lysosomal enzymes. Biochim. Biophys. Acta. 1241: 177-194]. This property has been shown to be exhibited by other mammalian receptors but not by the chicken and frog receptors. In a recent study however it was shown that the fish embryo MPR 300 binds human IGF-II. [Mendez, E., Planas, J.V., Castillo, J., Navarro, I. and Gutierrez, J. (2001) Identification of a type II insulin-like growth factor receptor in fish embryos. Endocrinology, 142: 1090-1097]. In the present study, we demonstrate that the purified goat and chicken liver receptors bind human IGF-II by employing cross-linking experiments (purified receptors and radiolabeled IGF-II) and by ligand blotting (using purified receptors and biotinylated IGF-II). Further CEF cells (chicken embryonic fibroblasts) that are known to contain the putative MPR 300 protein were employed to demonstrate that the CEF cell receptor binds human IGF-II.

Original languageEnglish (US)
Pages (from-to)101-112
Number of pages12
JournalBioscience Reports
Volume26
Issue number2
DOIs
StatePublished - Apr 2006

Fingerprint

IGF Type 2 Receptor
Insulin-Like Growth Factor II
Goats
Chickens
Proteins
Cell membranes
Fishes
Embryonic Structures
Fish
Cell Membrane
Endocrinology
Enzymes
Lysosomes
Mammals
Anura
Fibroblasts
Sorting
Liver
Ligands

Keywords

  • Biotinylated human-IGF-II
  • Human-IGF-II
  • Mannose 6-phosphate Receptor protein
  • MPR 300
  • Phosphomannan-Sepharose gel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Mannose 6-phosphate receptor (MPR 300) proteins from goat and chicken bind human IGF-II. / Koduru, Suresh; Yadavalli, Sivaramakrishna; Nadimpalli, Siva Kumar.

In: Bioscience Reports, Vol. 26, No. 2, 04.2006, p. 101-112.

Research output: Contribution to journalArticle

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