Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III

Laura C. Huang, Manfred Brockhaus, John L. Magnani, Frank Cuttitta, Steven Rosen, John D. Minna, Victor Ginsburg

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Monoclonal antibodies with an apparent specificity for human small-cell carcinoma, adenocarcinoma, and squamous carcinoma of the lung are produced by some hybridomas obtained from mice and rats immunized with an established line of human small cell lung cancer. Out of 85 of these antibodies produced by independently isolated hybridomas from 15 different fusions, 21 are directed against the sugar sequence which occurs in lacto-N-fucopentaose III ceramide, in several higher glycolipids and in glycoproteins. Specificity was determined by autoradiography of thin-layer chromatograms of glycolipids, by solid-phase radioimmunoassays, and by hapten inhibition studies. All 21 antibodies are of the immunoglobulin M type.

Original languageEnglish (US)
Pages (from-to)318-320
Number of pages3
JournalArchives of Biochemistry and Biophysics
Volume220
Issue number1
DOIs
StatePublished - 1983

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Glycolipids
Hybridomas
Sugars
Lung Neoplasms
Monoclonal Antibodies
Cells
Small Cell Carcinoma
Antibodies
Haptens
Ceramides
Small Cell Lung Carcinoma
Autoradiography
Radioimmunoassay
Immunoglobulin M
Rats
Squamous Cell Carcinoma
Glycoproteins
Adenocarcinoma
Fusion reactions
Lung

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III. / Huang, Laura C.; Brockhaus, Manfred; Magnani, John L.; Cuttitta, Frank; Rosen, Steven; Minna, John D.; Ginsburg, Victor.

In: Archives of Biochemistry and Biophysics, Vol. 220, No. 1, 1983, p. 318-320.

Research output: Contribution to journalArticle

Huang, Laura C. ; Brockhaus, Manfred ; Magnani, John L. ; Cuttitta, Frank ; Rosen, Steven ; Minna, John D. ; Ginsburg, Victor. / Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III. In: Archives of Biochemistry and Biophysics. 1983 ; Vol. 220, No. 1. pp. 318-320.
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