Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III

Laura C. Huang, Manfred Brockhaus, John L. Magnani, Frank Cuttitta, Steven Rosen, John D. Minna, Victor Ginsburg

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

Monoclonal antibodies with an apparent specificity for human small-cell carcinoma, adenocarcinoma, and squamous carcinoma of the lung are produced by some hybridomas obtained from mice and rats immunized with an established line of human small cell lung cancer. Out of 85 of these antibodies produced by independently isolated hybridomas from 15 different fusions, 21 are directed against the sugar sequence which occurs in lacto-N-fucopentaose III ceramide, in several higher glycolipids and in glycoproteins. Specificity was determined by autoradiography of thin-layer chromatograms of glycolipids, by solid-phase radioimmunoassays, and by hapten inhibition studies. All 21 antibodies are of the immunoglobulin M type.

Original languageEnglish (US)
Pages (from-to)318-320
Number of pages3
JournalArchives of Biochemistry and Biophysics
Volume220
Issue number1
DOIs
StatePublished - Jan 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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