Mapping of myogenin transcription during embryogenesis using transgenes linked to the myogenin control region

Tse Chang Cheng, Theresa A. Hanley, Jacqueline Mudd, John P. Merlie, Eric N. Olson

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

During vertebrate embryogenesis, the muscle-specific helix-loop-helix protein myogenin is expressed in muscle cell precursors in the developing somite myotome and limb bud before muscle fiber formation and is further upregulated during myogenesis. We show that cis-acting DNA sequences within the 5′ flanking region of the mouse myogenin gene are sufficient to direct appropriate temporal, spatial, and tissue-specific transcription of myogenin during mouse embryogenesis. Myogenin-lacZ transgenes trace the fate of embryonic cells that activate myogenin transcription and suggest that myogenic precursor cells that migrate from the somite myotome to the limb bud are committed to a myogenic fate in the absence of myogenin transcription. Activation of a myogenin-lacZ transgene can occur in limb bud explants in culture, indicating that signals required for activation of myogenin transcription are intrinsic to the limb bud and independent of other parts of the embryo. These results reveal multiple populations of myogenic precursor cells during development and suggest the existence of regulators other than myogenic helix-loop-helix proteins that maintain cells in the early limb bud in the myogenic lineage.

Original languageEnglish (US)
Pages (from-to)1649-1656
Number of pages8
JournalJournal of Cell Biology
Volume119
Issue number6
StatePublished - Dec 1992

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Myogenin
Transgenes
Embryonic Development
Limb Buds
Somites
Muscles
Muscle Development
Myoblasts
5' Flanking Region
Transcriptional Activation
Vertebrates
Proteins
Embryonic Structures

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mapping of myogenin transcription during embryogenesis using transgenes linked to the myogenin control region. / Cheng, Tse Chang; Hanley, Theresa A.; Mudd, Jacqueline; Merlie, John P.; Olson, Eric N.

In: Journal of Cell Biology, Vol. 119, No. 6, 12.1992, p. 1649-1656.

Research output: Contribution to journalArticle

Cheng, Tse Chang ; Hanley, Theresa A. ; Mudd, Jacqueline ; Merlie, John P. ; Olson, Eric N. / Mapping of myogenin transcription during embryogenesis using transgenes linked to the myogenin control region. In: Journal of Cell Biology. 1992 ; Vol. 119, No. 6. pp. 1649-1656.
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