TY - JOUR
T1 - Mapping of the human ribonuclease inhibitor gene (RNH) to chromosome 11p15 by in situ hybridization
AU - Zneimer, Susan M.
AU - Crawford, David
AU - Schneider, Nancy R.
AU - Beutler, Bruce
N1 - Funding Information:
The authors thank the technologists in the Cytogenetics Laho-ratory, Department of Pathology, UTSMCD, for their technical assistance in the chromosome preparations. This work was supported in part by NIH Grant ROlCA45525 and by a grant from the Tobacco Research Council. D. Crawford is supported by NIH Grant GM08014. While these studies were in progress, we were informed by Dr. B. Vallee that he and his colleagues had independently established the location of the human RNH gene using in situ hybridization techniques. Their assignment was also to chromosome 11~15.
PY - 1990/9
Y1 - 1990/9
N2 - Ribonuclease inhibitor (RNH) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization. A human placental cDNA was used to construct a 600-bp probe, which was then radiolabeled with tritium for in situ hybridization to human metaphase chromosomes. Localization of the RNH gene to 11p15, and possibly to 11p15.5, adds to a large number of genes assigned to this band, including 10 structural genes. This chromosomal region also represents an evolutionarily conserved syntenic group in the owl monkey, mouse, rat, and cow. Thus, regional assignment of RNH could facilitate the understanding of this gene and its association with ribonucleases, and perhaps extend a conserved syntenic region in mammalian genomes.
AB - Ribonuclease inhibitor (RNH) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization. A human placental cDNA was used to construct a 600-bp probe, which was then radiolabeled with tritium for in situ hybridization to human metaphase chromosomes. Localization of the RNH gene to 11p15, and possibly to 11p15.5, adds to a large number of genes assigned to this band, including 10 structural genes. This chromosomal region also represents an evolutionarily conserved syntenic group in the owl monkey, mouse, rat, and cow. Thus, regional assignment of RNH could facilitate the understanding of this gene and its association with ribonucleases, and perhaps extend a conserved syntenic region in mammalian genomes.
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U2 - 10.1016/0888-7543(90)90242-M
DO - 10.1016/0888-7543(90)90242-M
M3 - Article
C2 - 2081593
AN - SCOPUS:0025128152
SN - 0888-7543
VL - 8
SP - 175
EP - 178
JO - Genomics
JF - Genomics
IS - 1
ER -