Mapping the dynamics of force transduction at cell-cell junctions of epithelial clusters

Mei R osa Ng, Achim Besser, Joan S. Brugge, Gaudenz Danuser

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Force transduction at cell–cell adhesions regulates tissue development, maintenance and adaptation. We developed computational and experimental approaches to quantify, with both sub-cellular and multi-cellular resolution, the dynamics of force transmission in cell clusters. Applying this technology to spontaneously-forming adherent epithelial cell clusters, we found that basal force fluctuations were coupled to E-cadherin localization at the level of individual cell–cell junctions. At the multi-cellular scale, cell–cell force exchange depended on the cell position within a cluster, and was adaptive to reconfigurations due to cell divisions or positional rearrangements. Importantly, force transmission through a cell required coordinated modulation of cell-matrix adhesion and actomyosin contractility in the cell and its neighbors. These data provide insights into mechanisms that could control mechanical stress homeostasis in dynamic epithelial tissues, and highlight our methods as a resource for the study of mechanotransduction in cell–cell adhesions [corrected].

Original languageEnglish (US)
Pages (from-to)e03282
JournaleLife
Volume3
DOIs
StatePublished - 2014

Fingerprint

Intercellular Junctions
Adhesion
Tissue
Actomyosin
Tissue Adhesions
Cell-Matrix Junctions
Cadherins
Mechanical Stress
Cells
Modulation
Cell Division
Homeostasis
Epithelium
Epithelial Cells
Maintenance
Technology

Keywords

  • cell adhesion
  • cell biology
  • cell–cell interactions
  • cell–matrix interactions
  • intercellular adhesion molecules
  • mechanical forces
  • mechanotransduction
  • none

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mapping the dynamics of force transduction at cell-cell junctions of epithelial clusters. / Ng, Mei R osa; Besser, Achim; Brugge, Joan S.; Danuser, Gaudenz.

In: eLife, Vol. 3, 2014, p. e03282.

Research output: Contribution to journalArticle

Ng, Mei R osa ; Besser, Achim ; Brugge, Joan S. ; Danuser, Gaudenz. / Mapping the dynamics of force transduction at cell-cell junctions of epithelial clusters. In: eLife. 2014 ; Vol. 3. pp. e03282.
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