Mapping the idiotopes of a monoclonal anti-myoglobin antibody with syngeneic monoclonal anti-idiotypic antibodies

Detection of a common idiotope

H. Z. Streicher, F. Cuttitta, G. K. Buckenmeyer, H. Kawamura, J. Minna, J. A. Berzofsky

Research output: Contribution to journalArticle

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Abstract

A panel of syngeneic monoclonal anti-idiotypic antibodies was prepared by immunizing A.SW mice with keyhole limpet hemocyanin-coupled A.SW monoclonal anti-myoglobin (HAL 19, IgG1) and screening the cloned hybridomas for production of IgG2 binding to idiotype but not to certain other anti-myoglobin antibodies of the same subclass in an ELISA. With these antibodies, we identified three nonoverlapping idiotopes, based on three clusters of monoclonal anti-idiotopes that mutually inhibit within each cluster, but not between clusters (Cluster I: S2, S6, S8; Cluster II: S5, S7; Cluster III: S9). Only Cluster II antibodies block the binding of myoglobin to HAL 19 and so identify a binding site-related idiotope(s). Binding of both Cluster II monoclonals (S5 and S7) to Hal 19 is inhibited by a rabbit anti-idiotype that we previously reported detects a common cross-reactive anti-myoglobin idiotope in immune sera. However, only one of these, S7, and not S5, identifies an idiotope that is present on 20 to 30% of A.SW anti-myoglobin antibodies in immune sera and ascites. The panel of syngeneic monoclonal anti-idiotype antibodies also detects new idiotopes not detected by the rabbit anti-idiotype. The development of a panel of syngeneic monoclonal anti-idiotypic antibodies to different clusters of idiotopes on the same antibody molecule, including one that identifies a major common idiotope in immune sera, should allow the analysis of possible idiotype network regulation in vivo and in vitro in a completely syngeneic system.

Original languageEnglish (US)
Pages (from-to)1007-1014
Number of pages8
JournalJournal of Immunology
Volume136
Issue number3
StatePublished - 1986

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Myoglobin
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Immune Sera
Antibodies
Immunoglobulin G
Rabbits
S 6
Hybridomas
Ascites
Enzyme-Linked Immunosorbent Assay
Binding Sites

ASJC Scopus subject areas

  • Immunology

Cite this

Mapping the idiotopes of a monoclonal anti-myoglobin antibody with syngeneic monoclonal anti-idiotypic antibodies : Detection of a common idiotope. / Streicher, H. Z.; Cuttitta, F.; Buckenmeyer, G. K.; Kawamura, H.; Minna, J.; Berzofsky, J. A.

In: Journal of Immunology, Vol. 136, No. 3, 1986, p. 1007-1014.

Research output: Contribution to journalArticle

Streicher, H. Z. ; Cuttitta, F. ; Buckenmeyer, G. K. ; Kawamura, H. ; Minna, J. ; Berzofsky, J. A. / Mapping the idiotopes of a monoclonal anti-myoglobin antibody with syngeneic monoclonal anti-idiotypic antibodies : Detection of a common idiotope. In: Journal of Immunology. 1986 ; Vol. 136, No. 3. pp. 1007-1014.
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