MarC-V: A spreadsheet-based tool for analysis, normalization, and visualization of single cDNA microarray experiments

J. J. Schageman, M. Basit, T. D. Gallardo, H. R. Garner, R. V. Shohet

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The comprehensive analysis and visualization of data extracted from cDNA microarrays can be a time-consuming and error-prone process that becomes increasingly tedious with increased number of gene elements on a particular microarray. With the increasingly large number of gene elements on today's microarrays, analysis tools must be developed to meet this challenge. Here, we present MarC-V, a Microsoft® Excel® spreadsheet tool with Visual Basic® macros to automate much of the visualization and calculation involved in the analysis process while providing the familiarity and flexibility of Excel. Automated features of this tool include (i) lower-bound thresholding, (ii) data normalization, (iii) generation of ratio frequency distribution plots, (iv) generation of scatter plots color-coded by expression level, (v) ratio scoring based on intensity measurements, (vi) filtering of data based on expression level or specific gene interests, and (vii) exporting data for subsequent multi-array analysis. MarC-V also has an importing function included for GenePix® results (GPR) raw data files.

Original languageEnglish (US)
Pages (from-to)338-344
Number of pages7
JournalBioTechniques
Volume32
Issue number2
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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