Marker for type VI secretion system effectors

Dor Salomon, Lisa N. Kinch, David C. Trudgian, Xiaofeng Guo, John A. Klimko, Nick V. Grishin, Hamid Mirzaei, Kim Orth

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

Original languageEnglish (US)
Pages (from-to)9271-9276
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number25
DOIs
StatePublished - Jun 24 2014

Fingerprint

Proteins
Bacterial Proteins
Computational Biology
Proteomics
Virulence
Genome
Bacteria
Type VI Secretion Systems

Keywords

  • Bacterial competition
  • Colicin
  • Toxin
  • Vibrio

ASJC Scopus subject areas

  • General

Cite this

Marker for type VI secretion system effectors. / Salomon, Dor; Kinch, Lisa N.; Trudgian, David C.; Guo, Xiaofeng; Klimko, John A.; Grishin, Nick V.; Mirzaei, Hamid; Orth, Kim.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 25, 24.06.2014, p. 9271-9276.

Research output: Contribution to journalArticle

Salomon, Dor ; Kinch, Lisa N. ; Trudgian, David C. ; Guo, Xiaofeng ; Klimko, John A. ; Grishin, Nick V. ; Mirzaei, Hamid ; Orth, Kim. / Marker for type VI secretion system effectors. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 25. pp. 9271-9276.
@article{cb6148384eac4c64b2f544ba35b1c19b,
title = "Marker for type VI secretion system effectors",
abstract = "Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.",
keywords = "Bacterial competition, Colicin, Toxin, Vibrio",
author = "Dor Salomon and Kinch, {Lisa N.} and Trudgian, {David C.} and Xiaofeng Guo and Klimko, {John A.} and Grishin, {Nick V.} and Hamid Mirzaei and Kim Orth",
year = "2014",
month = "6",
day = "24",
doi = "10.1073/pnas.1406110111",
language = "English (US)",
volume = "111",
pages = "9271--9276",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "25",

}

TY - JOUR

T1 - Marker for type VI secretion system effectors

AU - Salomon, Dor

AU - Kinch, Lisa N.

AU - Trudgian, David C.

AU - Guo, Xiaofeng

AU - Klimko, John A.

AU - Grishin, Nick V.

AU - Mirzaei, Hamid

AU - Orth, Kim

PY - 2014/6/24

Y1 - 2014/6/24

N2 - Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

AB - Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

KW - Bacterial competition

KW - Colicin

KW - Toxin

KW - Vibrio

UR - http://www.scopus.com/inward/record.url?scp=84903439619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903439619&partnerID=8YFLogxK

U2 - 10.1073/pnas.1406110111

DO - 10.1073/pnas.1406110111

M3 - Article

VL - 111

SP - 9271

EP - 9276

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 25

ER -