Mass enucleation of tissue culture cell monolayers

Andrew H. Crenshaw, Jerry W. Shay, Leonard R. Murrell

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm2 culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.

Original languageEnglish (US)
Pages (from-to)127-130
Number of pages4
JournalJournal of Tissue Culture Methods
Volume6
Issue number3-4
DOIs
StatePublished - Sep 1980

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Keywords

  • cell enucleation
  • cytochalasin B
  • human fibroblasts

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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