Mass enucleation of tissue culture cell monolayers

Andrew H. Crenshaw; Jerry W. Shay; Leonard R. Murrell

doi:10.1007/BF02082864

Mass enucleation of tissue culture cell monolayers

Andrew H. Crenshaw, Jerry W. Shay, Leonard R. Murrell

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm² culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.

Original language	English (US)
Pages (from-to)	127-130
Number of pages	4
Journal	Journal of Tissue Culture Methods
Volume	6
Issue number	3-4
DOIs	https://doi.org/10.1007/BF02082864
State	Published - Sep 1980

Keywords

cell enucleation
cytochalasin B
human fibroblasts

ASJC Scopus subject areas

Anatomy
Pathology and Forensic Medicine

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10.1007/BF02082864

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title = "Mass enucleation of tissue culture cell monolayers",

abstract = "A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm2 culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.",

keywords = "cell enucleation, cytochalasin B, human fibroblasts",

author = "Crenshaw, {Andrew H.} and Shay, {Jerry W.} and Murrell, {Leonard R.}",

year = "1980",

month = sep,

doi = "10.1007/BF02082864",

language = "English (US)",

volume = "6",

pages = "127--130",

journal = "Journal of Tissue Culture Methods",

issn = "0271-8057",

publisher = "Springer Netherlands",

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T1 - Mass enucleation of tissue culture cell monolayers

AU - Crenshaw, Andrew H.

AU - Shay, Jerry W.

AU - Murrell, Leonard R.

PY - 1980/9

Y1 - 1980/9

N2 - A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm2 culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.

AB - A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm2 culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.

KW - cell enucleation

KW - cytochalasin B

KW - human fibroblasts

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U2 - 10.1007/BF02082864

DO - 10.1007/BF02082864

M3 - Article

AN - SCOPUS:0344230102

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EP - 130

JO - Journal of Tissue Culture Methods

JF - Journal of Tissue Culture Methods

IS - 3-4

ER -

Mass enucleation of tissue culture cell monolayers

Abstract

Keywords

ASJC Scopus subject areas

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