Mass enucleation of tissue culture cell monolayers

Andrew H. Crenshaw, Jerry W. Shay, Leonard R. Murrell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A method is described for cytochalasin B induced enucleation of cultured mammalian cells grown in monolayers and centrifuged in 75 cm2 culture flasks. Enucleation yields of approximately 90% from normal human fibroblasts can be achieved routinely with a single centrifugation in the presence of 10 μg/ml cytochalasin B. No loss of cells due to flask breakage occurs at the maximum recommended rotor speed, and flasks can be successfully centrifuged several times.

Original languageEnglish (US)
Pages (from-to)127-130
Number of pages4
JournalJournal of Tissue Culture Methods
Volume6
Issue number3-4
DOIs
StatePublished - Sep 1980

Fingerprint

Tissue culture
Cytochalasin B
Centrifugation
Fibroblasts
Monolayers
Cell Culture Techniques
Rotors
Cells
Cultured Cells

Keywords

  • cell enucleation
  • cytochalasin B
  • human fibroblasts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Mass enucleation of tissue culture cell monolayers. / Crenshaw, Andrew H.; Shay, Jerry W.; Murrell, Leonard R.

In: Journal of Tissue Culture Methods, Vol. 6, No. 3-4, 09.1980, p. 127-130.

Research output: Contribution to journalArticle

Crenshaw, Andrew H. ; Shay, Jerry W. ; Murrell, Leonard R. / Mass enucleation of tissue culture cell monolayers. In: Journal of Tissue Culture Methods. 1980 ; Vol. 6, No. 3-4. pp. 127-130.
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