Mass spectrometry imaging and identification of peptides associated with cephalic ganglia regeneration in Schmidtea mediterranea

Ta Hsuan Ong, Elena V. Romanova, Rachel H. Roberts-Galbraith, Ning Yang, Tyler A. Zimmerman, James J. Collins, Ji Eun Lee, Neil L. Kelleher, Phillip A. Newmark, Jonathan V. Sweedler

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tissue regeneration is a complex process that involves a mosaic of molecules that vary spatially and temporally. Insights into the chemical signaling underlying this process can be achieved with a multiplex and untargeted chemical imaging method such as mass spectrometry imaging (MSI), which can enable de novo studies of nervous system regeneration. A combination of MSI and multivariate statistics was used to differentiate peptide dynamics in the freshwater planarian flatworm Schmidtea mediterranea at different time points during cephalic ganglia regeneration. A protocol was developed to make S. mediterranea tissues amenable for MSI. MS ion images of planarian tissue sections allow changes in peptides and unknown compounds to be followed as a function of cephalic ganglia regeneration. In conjunction with fluorescence imaging, our results suggest that even though the cephalic ganglia structure is visible after 6 days of regeneration, the original chemical composition of these regenerated structures is regained only after 12 days. Differences were observed in many peptides, such as those derived from secreted peptide 4 and EYE53-1. Peptidomic analysis further identified multiple peptides from various known prohormones, histone proteins, and DNA- and RNAbinding proteins as being associated with the regeneration process. Mass spectrometry data also facilitated the identification of a new prohormone, which we have named secreted peptide prohormone 20 (SPP-20), and is up-regulated during regeneration in planarians.

Original languageEnglish (US)
Pages (from-to)8109-8120
Number of pages12
JournalJournal of Biological Chemistry
Volume291
Issue number15
DOIs
StatePublished - Apr 8 2016

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Ganglia
Mass spectrometry
Regeneration
Mass Spectrometry
Head
Imaging techniques
Planarians
Peptides
Tissue
Tissue regeneration
Platyhelminths
Neurology
Optical Imaging
Histones
Fresh Water
Proteins
Nervous System
Fluorescence
Statistics
Ions

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Mass spectrometry imaging and identification of peptides associated with cephalic ganglia regeneration in Schmidtea mediterranea. / Ong, Ta Hsuan; Romanova, Elena V.; Roberts-Galbraith, Rachel H.; Yang, Ning; Zimmerman, Tyler A.; Collins, James J.; Lee, Ji Eun; Kelleher, Neil L.; Newmark, Phillip A.; Sweedler, Jonathan V.

In: Journal of Biological Chemistry, Vol. 291, No. 15, 08.04.2016, p. 8109-8120.

Research output: Contribution to journalArticle

Ong, TH, Romanova, EV, Roberts-Galbraith, RH, Yang, N, Zimmerman, TA, Collins, JJ, Lee, JE, Kelleher, NL, Newmark, PA & Sweedler, JV 2016, 'Mass spectrometry imaging and identification of peptides associated with cephalic ganglia regeneration in Schmidtea mediterranea', Journal of Biological Chemistry, vol. 291, no. 15, pp. 8109-8120. https://doi.org/10.1074/jbc.M115.709196
Ong, Ta Hsuan ; Romanova, Elena V. ; Roberts-Galbraith, Rachel H. ; Yang, Ning ; Zimmerman, Tyler A. ; Collins, James J. ; Lee, Ji Eun ; Kelleher, Neil L. ; Newmark, Phillip A. ; Sweedler, Jonathan V. / Mass spectrometry imaging and identification of peptides associated with cephalic ganglia regeneration in Schmidtea mediterranea. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 15. pp. 8109-8120.
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