TY - JOUR
T1 - Math1-driven GFP expression in the developing nervous system of transgenic mice
AU - Lumpkin, Ellen A.
AU - Collisson, Tandi
AU - Parab, Preeti
AU - Omer-Abdalla, Adil
AU - Haeberle, Henry
AU - Chen, Ping
AU - Doetzlhofer, Angelika
AU - White, Patricia
AU - Groves, Andrew
AU - Segil, Neil
AU - Johnson, Jane E.
N1 - Funding Information:
We gratefully acknowledge Drs K. Lee and T. M. Jessell for providing LH2A/B antisera, and the Developmental Studies Hybridoma Bank for TAG-1 specific antibodies (4D7). E. A. L. is supported by the Sandler Program in Basic Sciences. H. H. is a NSF predoctoral fellow. P. C. is supported by the NIH. A. D., P. W., A. G. and N. S. are supported by the NIH and the National Organization for Hearing Research. T. C, P. P., and A. O.-A. are supported by grants from the March of Dimes and the Texas Education Coordinating Board to J.E.J.
PY - 2003/8
Y1 - 2003/8
N2 - Math1 is a bHLH transcription factor expressed in neural progenitor cells in multiple regions of the nervous system. Previously we identified a Math1 enhancer that directs expression of reporter genes in a Math1 specific pattern [Development 127 (2000) 1185]. We have used a portion of this enhancer to drive expression of a nuclear GFP reporter in the Math1 lineage in transgenic mice. In this transgenic mouse strain, GFP is expressed in Math1 domains in the (1) developing spinal cord in progenitors to dI1 dorsal interneurons, (2) granule-cell progenitors in the developing cerebellum, (3) Merkel cells in the skin, and (4) hair cells in the developing vestibular and auditory systems. Furthermore, non-Math1 related expression is detected that is likely due to the absence of inhibitory regulatory sequences from the transgene. These expression domains include (1) the apical ectodermal ridge in developing limbs, (2) post-mitotic cells in the developing cortex and spinal cord, (3) the dentate gyrus, (4) retina, and (5) olfactory epithelium. Because GFP marks specific neuronal cell types in living tissue, this transgenic strain is a powerful tool for future studies on the development and electrophysiological properties of distinct cell types in the central nervous system and in sensory systems.
AB - Math1 is a bHLH transcription factor expressed in neural progenitor cells in multiple regions of the nervous system. Previously we identified a Math1 enhancer that directs expression of reporter genes in a Math1 specific pattern [Development 127 (2000) 1185]. We have used a portion of this enhancer to drive expression of a nuclear GFP reporter in the Math1 lineage in transgenic mice. In this transgenic mouse strain, GFP is expressed in Math1 domains in the (1) developing spinal cord in progenitors to dI1 dorsal interneurons, (2) granule-cell progenitors in the developing cerebellum, (3) Merkel cells in the skin, and (4) hair cells in the developing vestibular and auditory systems. Furthermore, non-Math1 related expression is detected that is likely due to the absence of inhibitory regulatory sequences from the transgene. These expression domains include (1) the apical ectodermal ridge in developing limbs, (2) post-mitotic cells in the developing cortex and spinal cord, (3) the dentate gyrus, (4) retina, and (5) olfactory epithelium. Because GFP marks specific neuronal cell types in living tissue, this transgenic strain is a powerful tool for future studies on the development and electrophysiological properties of distinct cell types in the central nervous system and in sensory systems.
KW - AER
KW - Cerebellar granule cells
KW - Cerebral cortex
KW - Cochlea
KW - Commissural interneurons
KW - Dentate gyrus
KW - Dorsal neural tube
KW - Hair cells
KW - Mechanoreceptor
KW - Merkel cell
KW - Olfactory epithelium
KW - Retina
KW - Vestibular sensory cells
KW - bHLH transcription factor
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U2 - 10.1016/S1567-133X(03)00089-9
DO - 10.1016/S1567-133X(03)00089-9
M3 - Article
C2 - 12915300
AN - SCOPUS:12444259658
SN - 1567-133X
VL - 3
SP - 389
EP - 395
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 4
ER -