Matrix metalloproteinases and tumor necrosis factor α in glaucomatous optic nerve head

Xiaoming Yan, Gülgün Tezel, Martin B. Wax, Deepak P. Edward

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Objective: To study expression and location of matrix metalloproteinases (MMPs) and tumor necrosis factor α (TNF-α) in glaucomatous optic nerve heads, which are known to be secreted in response to a variety of neuronal injury. Methods: Four postmortem eyes from patients with primary open-angle glaucoma, 7 eyes from patients with normal-pressure glaucoma, and 4 eyes from age-matched normal donors were studied by immunohistochemistry. The sections of the optic nerve heads were examined after immunostaining with antibodies to MMPs (MMP-1, MMP-2, and MMP-3), TNF-α, or TNF-α receptor 1. Results: The intensity of the immunostaining and the number of stained cells for MMPs, TNF-α, or TNF-α receptor 1 were greater in the glaucomatous optic nerve heads, particularly in eyes with normal-pressure glaucoma compared with age- matched controls. Positive immunostaining was observed in all regions of the glaucomatous optic nerve heads, but most prominently in the postlaminar region. Immunostaining was observed mainly in glial cells and their processes around the axons and blood vessels and in pial septae. Conclusion: There is increased immunostaining for MMPs, TNF-α and TNF-α receptor i in the glaucomatous optic nerve head, which suggests increased expression of these proteins in glaucoma and thereby implies a role in the tissue remodeling and degenerative changes seen in glaucomatous optic nerve heads. Clinical Relevance: The MMPs and TNF-α may be components of astroglial activation that occurs in glaucomatous optic nerve heads. The biological alterations in the expression of these proteins may pay a role in the progression of glaucomatous optic neuropathy.

Original languageEnglish (US)
Pages (from-to)666-673
Number of pages8
JournalArchives of Ophthalmology
Volume118
Issue number5
StatePublished - May 2000

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Optic Disk
Matrix Metalloproteinases
Tumor Necrosis Factor-alpha
Tumor Necrosis Factor Receptors
Glaucoma
Pressure
Matrix Metalloproteinase 3
Optic Nerve Diseases
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Neuroglia
Blood Vessels
Axons
Proteins
Cell Count
Immunohistochemistry
Tissue Donors
Antibodies
Wounds and Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Yan, X., Tezel, G., Wax, M. B., & Edward, D. P. (2000). Matrix metalloproteinases and tumor necrosis factor α in glaucomatous optic nerve head. Archives of Ophthalmology, 118(5), 666-673.

Matrix metalloproteinases and tumor necrosis factor α in glaucomatous optic nerve head. / Yan, Xiaoming; Tezel, Gülgün; Wax, Martin B.; Edward, Deepak P.

In: Archives of Ophthalmology, Vol. 118, No. 5, 05.2000, p. 666-673.

Research output: Contribution to journalArticle

Yan, X, Tezel, G, Wax, MB & Edward, DP 2000, 'Matrix metalloproteinases and tumor necrosis factor α in glaucomatous optic nerve head', Archives of Ophthalmology, vol. 118, no. 5, pp. 666-673.
Yan, Xiaoming ; Tezel, Gülgün ; Wax, Martin B. ; Edward, Deepak P. / Matrix metalloproteinases and tumor necrosis factor α in glaucomatous optic nerve head. In: Archives of Ophthalmology. 2000 ; Vol. 118, No. 5. pp. 666-673.
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