Measurement of anionic sites on the surfaces of baby hamster kidney cells using radiolabeled polycationic ferritin

Frederick Grinnell, Donald C. Hays

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4 Citations (Scopus)

Abstract

A method is described to determine relative numbers of anionic sites on the surfaces of cells under physiological conditions by binding studies with radiolabeled polycationic ferritin. Labeling of cells by polycationic ferritin occurred very rapidly even at 2°C and was essentially complete within 1 min. At 22°C, a rapid initial phase of labeling was followed by a second, slower binding phase. The interaction of rapidly labeled cell surface anionic sites with polycationic ferritin had a binding constant of 3.6 × 106m-1 (measured at 2°C) and there were about 4 × 106 of these sites per cell.

Original languageEnglish (US)
Pages (from-to)400-402
Number of pages3
JournalAnalytical Biochemistry
Volume97
Issue number2
DOIs
StatePublished - Sep 1 1979

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Cricetinae
Kidney
Labeling
polycationic ferritin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Measurement of anionic sites on the surfaces of baby hamster kidney cells using radiolabeled polycationic ferritin. / Grinnell, Frederick; Hays, Donald C.

In: Analytical Biochemistry, Vol. 97, No. 2, 01.09.1979, p. 400-402.

Research output: Contribution to journalArticle

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