Abstract
A method is described to determine relative numbers of anionic sites on the surfaces of cells under physiological conditions by binding studies with radiolabeled polycationic ferritin. Labeling of cells by polycationic ferritin occurred very rapidly even at 2°C and was essentially complete within 1 min. At 22°C, a rapid initial phase of labeling was followed by a second, slower binding phase. The interaction of rapidly labeled cell surface anionic sites with polycationic ferritin had a binding constant of 3.6 × 106m-1 (measured at 2°C) and there were about 4 × 106 of these sites per cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 400-402 |
| Number of pages | 3 |
| Journal | Analytical Biochemistry |
| Volume | 97 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 1 1979 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Measurement of anionic sites on the surfaces of baby hamster kidney cells using radiolabeled polycationic ferritin. / Grinnell, Frederick; Hays, Donald C.
In: Analytical Biochemistry, Vol. 97, No. 2, 01.09.1979, p. 400-402.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of anionic sites on the surfaces of baby hamster kidney cells using radiolabeled polycationic ferritin
AU - Grinnell, Frederick
AU - Hays, Donald C.
PY - 1979/9/1
Y1 - 1979/9/1
N2 - A method is described to determine relative numbers of anionic sites on the surfaces of cells under physiological conditions by binding studies with radiolabeled polycationic ferritin. Labeling of cells by polycationic ferritin occurred very rapidly even at 2°C and was essentially complete within 1 min. At 22°C, a rapid initial phase of labeling was followed by a second, slower binding phase. The interaction of rapidly labeled cell surface anionic sites with polycationic ferritin had a binding constant of 3.6 × 106m-1 (measured at 2°C) and there were about 4 × 106 of these sites per cell.
AB - A method is described to determine relative numbers of anionic sites on the surfaces of cells under physiological conditions by binding studies with radiolabeled polycationic ferritin. Labeling of cells by polycationic ferritin occurred very rapidly even at 2°C and was essentially complete within 1 min. At 22°C, a rapid initial phase of labeling was followed by a second, slower binding phase. The interaction of rapidly labeled cell surface anionic sites with polycationic ferritin had a binding constant of 3.6 × 106m-1 (measured at 2°C) and there were about 4 × 106 of these sites per cell.
UR - http://www.scopus.com/inward/record.url?scp=0018717155&partnerID=8YFLogxK
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U2 - 10.1016/0003-2697(79)90092-7
DO - 10.1016/0003-2697(79)90092-7
M3 - Article
C2 - 525801
AN - SCOPUS:0018717155
VL - 97
SP - 400
EP - 402
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -