Measurement of anionic sites on the surfaces of baby hamster kidney cells using radiolabeled polycationic ferritin

Frederick Grinnell, Donald C. Hays

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method is described to determine relative numbers of anionic sites on the surfaces of cells under physiological conditions by binding studies with radiolabeled polycationic ferritin. Labeling of cells by polycationic ferritin occurred very rapidly even at 2°C and was essentially complete within 1 min. At 22°C, a rapid initial phase of labeling was followed by a second, slower binding phase. The interaction of rapidly labeled cell surface anionic sites with polycationic ferritin had a binding constant of 3.6 × 106m-1 (measured at 2°C) and there were about 4 × 106 of these sites per cell.

Original languageEnglish (US)
Pages (from-to)400-402
Number of pages3
JournalAnalytical biochemistry
Volume97
Issue number2
DOIs
StatePublished - Sep 1 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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