Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis

D. C. Metrikin, C. A. Wilson, B. A. Berkowitz, M. K. Lam, G. K. Wood, Ronald M Peshock

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Purpose. Endophthalmitis is a severe inflammatory disorder with profound visual consequences. Treatment of this disorder has been limited by the lack of quantitative information regarding retinal responses to severe inflammation. The purpose of this study was to measure the effect of endotoxin-induced endophthalmitis on blood-retinal barrier (BRB) function in vivo rising contrast-enhanced magnetic resonance imaging (MRI). Methods. Endophthalmitis was produced by injecting Escherichia coli endotoxin into the mid-vitreous of pigmented rabbits. Contrast-enhanced MRI was performed at selected intervals thereafter. In all cases, a clinical grading system was used to assess the severity of inflammation before imaging. In a dose- response experiment, total vitreous protein was measured from vitreous specimens obtained 1 day after endotoxin injection and immediately after the imaging procedure. Results. At 1 day after injection, endotoxin produced a selective breakdown of the inner BRB at all doses evaluated (0.01 μg to 500 μg). Permeability-surface area product normalized to the area of leaky retina (PS') increased from 1.35 ± 0.78 x 10-4 cm/minute (mean ± SEM, n = 4 eyes) at a dose of 0.01 μg to 8.15 ± 2.22 x 10-4 cm/minute (n = 4 eyes) at a dose of 10 μg. Inner BRB integrity was restored by day 28 after injection. In general, changes in PS', blood-aqueous barrier leakage, mean clinical score, and vitreous protein concentration were found, but the correlation between any two of these parameters was poor. Conclusion. Leakage of contrast appears early in the course of endotoxin-induced endophthalmitis and is a self-limited process. In future studies, these quantifiable changes in BRB permeability should prove useful in the assessment of various therapeutic interventions.

Original languageEnglish (US)
Pages (from-to)1361-1370
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number7
StatePublished - 1995

Fingerprint

Blood-Retinal Barrier
Endophthalmitis
Endotoxins
Injections
Permeability
Blood-Aqueous Barrier
Magnetic Resonance Imaging
Inflammation
Retina
Proteins
Rabbits
Therapeutics

Keywords

  • blood-aqueous barrier
  • blood-retinal barrier
  • endophthalmitis
  • endotoxin
  • magnetic resonance imaging
  • rabbit eye

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Metrikin, D. C., Wilson, C. A., Berkowitz, B. A., Lam, M. K., Wood, G. K., & Peshock, R. M. (1995). Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis. Investigative Ophthalmology and Visual Science, 36(7), 1361-1370.

Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis. / Metrikin, D. C.; Wilson, C. A.; Berkowitz, B. A.; Lam, M. K.; Wood, G. K.; Peshock, Ronald M.

In: Investigative Ophthalmology and Visual Science, Vol. 36, No. 7, 1995, p. 1361-1370.

Research output: Contribution to journalArticle

Metrikin, DC, Wilson, CA, Berkowitz, BA, Lam, MK, Wood, GK & Peshock, RM 1995, 'Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis', Investigative Ophthalmology and Visual Science, vol. 36, no. 7, pp. 1361-1370.
Metrikin, D. C. ; Wilson, C. A. ; Berkowitz, B. A. ; Lam, M. K. ; Wood, G. K. ; Peshock, Ronald M. / Measurement of blood-retinal barrier breakdown in endotoxin-induced endophthalmitis. In: Investigative Ophthalmology and Visual Science. 1995 ; Vol. 36, No. 7. pp. 1361-1370.
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