Measurement of corneal sublayer thickness and transparency in transgenic mice with altered corneal clarity using in vivo confocal microscopy

James V. Jester, Young Ghee Lee, Jie Li, Shukti Chakravarti, Jennifer Paul, Walter M Petroll, Harrison D Cavanagh

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Measurement of sublayer thickness and transparency at cellular level in the living animal are critical to understanding the role of specific transgenes and transgene products in controlling corneal development and maintenance of transparency. Using two different transgenic mouse strains having altered corneal clarity, we have evaluated the ability of in vivo confocal microscopy to measure corneal haze and localize light scattering structures. Projection of 2-D and 3-D image information identified the nature and location of light scattering within the cornea and allowed correlation of unique structural differences to transgene expression. Our findings suggest that in vivo confocal microscopy can be used to identify the effects of transgene expression on mouse corneal transparency.

Original languageEnglish (US)
Pages (from-to)1283-1290
Number of pages8
JournalVision Research
Volume41
Issue number10-11
DOIs
StatePublished - May 5 2001

Keywords

  • Confocal microscopy
  • Cornea
  • Digital imaging
  • Transgenic
  • Transparency

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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