Measurement of gluconeogenesis and pyruvate recycling in the rat liver: A simple analysis of glucose and glutamate isotopomers during metabolism of [1,2,3-13C3]propionate

John G. Jones, Randy Naidoo, A. Dean Sherry, F. M H Jeffrey, G. Larry Cottam, Craig R. Malloy

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Simple equations that relate glucose and glutamate 13C-NMR multiplet areas to gluconeogenesis and pyruvate recycling during metabolism of [1,2,3-13C3]propionate are presented. In isolated rat livers, gluconeogenic flux was 1.2 times TCA cycle flux and about 40% of the oxaloacetate pool underwent recycling to pyruvate prior to formation of glucose. The 13C spectra of glucose collected from rats after gastric versus intravenous administration of [1,2,3-13C3]propionate indicated that pyruvate recycling was slightly higher in vivo (49%) while glucose production was unchanged. This indicates that a direct measure of gluconeogenesis and pyruvate recycling may be obtained from a single 13C-NMR spectrum of blood collected after oral administration of enriched propionate.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalFEBS Letters
Volume412
Issue number1
DOIs
StatePublished - Jul 21 1997

Keywords

  • C isotopomer analysis
  • Gluconeogenesis
  • Pyruvate recycling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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