Measurement of glycine in gray and white matter in the human brain in vivo by 1H MRS at 7.0 T

Abhishek Banerjee, Sandeep Ganji, Keith Hulsey, Ivan Dimitrov, Elizabeth Maher, Subroto Ghose, Carol Tamminga, Changho Choi

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The concentration of glycine (Gly) was measured in gray matter (GM) and white matter (WM) in the human brain using single-voxel localized 1H MRS at 7 T. A point-resolved spectroscopy sequence with echo time = 150 ms was used for measuring Gly levels in various regions of the frontal and occipital lobes in 11 healthy volunteers and one subject with a glioblastoma. The point-resolved spectroscopy spectra were analyzed with LCModel using basis functions generated from density matrix simulations that included the effects of volume localized radio-frequency and gradient pulses. The fraction of GM and white matter within the voxels was obtained from T1-weighted image segmentation. The metabolite concentrations within the voxels, estimated with respect to the GM + WM water concentrations, were fitted to a linear function of fractional GM content. The Gly concentrations in pure GM and white matter were estimated to be 1.1 and 0.1 mM, with 95% confidence intervals 1.0-1.2 and 0.0-0.2, respectively. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)325-331
Number of pages7
JournalMagnetic resonance in medicine
Volume68
Issue number2
DOIs
StatePublished - Aug 2012

Keywords

  • 1H MRS
  • 3T
  • Glycine
  • gray matter
  • human brain
  • point-resolved spectroscopy (PRESS)
  • white matter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Measurement of glycine in gray and white matter in the human brain in vivo by 1H MRS at 7.0 T'. Together they form a unique fingerprint.

Cite this