Measurement of glycine in human prefrontal brain by point-resolved spectroscopy at 7.0 tesla in vivo

Changho Choi, Deborah Douglas, Halima Hawesa, Ashish Jindal, Charles Storey, Ivan Dimitrov

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Measurement of glycine in human frontal brain by an optimized point-resolved spectroscopy sequence at 7 T is reported. Echo time dependencies of the overlapping coupled resonances of myo-inositol, free choline, and threonine were investigated with density matrix simulations, incorporating the slice-selective radiofrequency and gradient pulses. The numerical simulations indicated that the selectivity of the 3.55-ppm glycine singlet is maximized at (TE1, TE2) = (101, 51) ms. Phantom experiments indicated that the myo-inositol peak amplitude between 3.5 and 3.6 ppm is reduced by a factor of 30 following the optimized point-resolved spectroscopy, as predicted by the simulation. From LCModel analyses, the glycine concentration in the medial prefrontal cortex in healthy adults was estimated, with a mean Cramér-Rao lower bound of 7 ± 1% (mean ± standard deviation; n = 7), to be 0.8 ± 0.1 mM, with reference to total creatine at 8 mM.

Original languageEnglish (US)
Pages (from-to)1305-1310
Number of pages6
JournalMagnetic resonance in medicine
Volume62
Issue number5
DOIs
StatePublished - Nov 1 2009

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Keywords

  • 7 T
  • Glycine
  • H-MRS
  • Human brain
  • PRESS

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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