Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

Jeffrey B. Guild, Chris Xu, Watt W. Webb

Research output: Contribution to journalArticle

87 Scopus citations


We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.

Original languageEnglish (US)
Pages (from-to)397-401
Number of pages5
JournalApplied Optics
Issue number1
StatePublished - Jan 1 1997


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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