We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Engineering (miscellaneous)
- Electrical and Electronic Engineering