Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

E. H. Radany; E. C. Friedberg

doi:10.1128/jvi.47.2.367-369.1983

Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

Research output: Contribution to journal › Article › peer-review

Abstract

Escherichia coli uvr^- cells, prelabeled in their DNA, were infected with phage T4 denV⁺ or T4 denV^- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

Original language	English (US)
Pages (from-to)	367-369
Number of pages	3
Journal	Journal of virology
Volume	47
Issue number	2
DOIs	https://doi.org/10.1128/jvi.47.2.367-369.1983
State	Published - 1983

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

Access to Document

10.1128/jvi.47.2.367-369.1983

Cite this

@article{329495441dbd427bbb11214db7ef9463,

title = "Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo",

abstract = "Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.",

author = "Radany, {E. H.} and Friedberg, {E. C.}",

year = "1983",

doi = "10.1128/jvi.47.2.367-369.1983",

language = "English (US)",

volume = "47",

pages = "367--369",

journal = "Journal of virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

AU - Radany, E. H.

AU - Friedberg, E. C.

PY - 1983

Y1 - 1983

N2 - Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

AB - Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0020585274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020585274&partnerID=8YFLogxK

U2 - 10.1128/jvi.47.2.367-369.1983

DO - 10.1128/jvi.47.2.367-369.1983

M3 - Article

C2 - 6352957

AN - SCOPUS:0020585274

SN - 0022-538X

VL - 47

SP - 367

EP - 369

JO - Journal of virology

JF - Journal of virology

IS - 2

ER -

Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this