Measurement of repair patch size by quantitation of nucleotides excised during DNA repair in vivo

E. H. Radany, E. C. Friedberg

Research output: Contribution to journalArticle

Abstract

Escherichia coli uvr- cells, prelabeled in their DNA, were infected with phage T4 denV+ or T4 denV- under conditions that preclude phage-mediated degradation of the bacterial chromosome. Measurement of the distribution of acid-soluble radioactivity between pyrimidine dimers and nondimer nucleotides in cell extracts yielded calculated estimates of the average size of excision repair tracts that are in good agreement with the size of repair patches determined by others using direct measurement of repair synthesis.

Original languageEnglish (US)
Pages (from-to)367-369
Number of pages3
JournalJournal of virology
Volume47
Issue number2
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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