Measurement of the temperature of the resting rotor in analytical ultracentrifugation

Rodolfo Ghirlando, Huaying Zhao, Andrea Balbo, Grzegorz Piszczek, Ute Curth, Chad A Brautigam, Peter Schuck

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2 °C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.

Original languageEnglish (US)
Pages (from-to)37-39
Number of pages3
JournalAnalytical biochemistry
Volume458
DOIs
StatePublished - Aug 1 2014

Keywords

  • Hydrodynamics
  • Sedimentation velocity
  • Temperature calibration

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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