Mechanical properties of acellular peripheral nerve

Gregory H. Borschel, Kevin F. Kia, William M. Kuzon, Robert G. Dennis

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Background. Acellular nerve has been used in experimental models as a peripheral nerve substitute. Our objective was to determine the difference in tensile strength between fresh and chemically treated acellularized peripheral nerve. Materials and methods. F344 rat sciatic nerves were either fresh or acellularized and tested either whole (Part A) or transected and repaired (Part B). For all constructs, the mean ultimate stress, mean ultimate strain, Young's modulus, and total mechanical work to fracture were calculated. Results. The average ultimate strains for Groups A-1 and A-2 were 0.480 ± 0.117 and 0.810 ± 0.114, respectively. The Young's moduli in Groups A-1 and A-2 were 576 ± 160 and 580 ± 150 kPa, respectively. In Groups A-1 and A-2, the normalized work to failure was 0.35 ± 0.14 and 1.11 ± 0.38 N. The specimens in Group B-1 withstood an average ultimate stress of 780 ± 280 kPa. The specimens in Group B-2 withstood an average ultimate stress of 405 ± 20 kPa. The average ultimate strains for Groups B-1 and B-2 were 0.319 ± 0.087 and 0.266 ± 0.019, respectively. The Young's moduli in Groups B-1 and B-2 were 4,030 ± 1360 and 2,290 ± 280 kPa, respectively. The normalized work to failure in Groups B-1 and B-2 was calculated as 0.22 ± 0.04 and 0.11 ± 0.02 N. Conclusions. Although adequately robust for reconstructive procedures, the acellular peripheral nerve had decreased tensile strength compared with fresh nerve either when tested whole or when transected and repaired.

Original languageEnglish (US)
Pages (from-to)133-139
Number of pages7
JournalJournal of Surgical Research
Volume114
Issue number2
DOIs
StatePublished - Oct 2003

Fingerprint

Elastic Modulus
Peripheral Nerves
varespladib methyl
Tensile Strength
Inbred F344 Rats
Sciatic Nerve
Theoretical Models

Keywords

  • Acellular nerve
  • Biomechanics
  • Extracellular matrix
  • Mechanical properties
  • Nerve graft
  • Nerve regeneration
  • Nerve repair
  • Peripheral nerve
  • Tensile strength
  • Tissue engineering

ASJC Scopus subject areas

  • Surgery

Cite this

Mechanical properties of acellular peripheral nerve. / Borschel, Gregory H.; Kia, Kevin F.; Kuzon, William M.; Dennis, Robert G.

In: Journal of Surgical Research, Vol. 114, No. 2, 10.2003, p. 133-139.

Research output: Contribution to journalArticle

Borschel, Gregory H. ; Kia, Kevin F. ; Kuzon, William M. ; Dennis, Robert G. / Mechanical properties of acellular peripheral nerve. In: Journal of Surgical Research. 2003 ; Vol. 114, No. 2. pp. 133-139.
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abstract = "Background. Acellular nerve has been used in experimental models as a peripheral nerve substitute. Our objective was to determine the difference in tensile strength between fresh and chemically treated acellularized peripheral nerve. Materials and methods. F344 rat sciatic nerves were either fresh or acellularized and tested either whole (Part A) or transected and repaired (Part B). For all constructs, the mean ultimate stress, mean ultimate strain, Young's modulus, and total mechanical work to fracture were calculated. Results. The average ultimate strains for Groups A-1 and A-2 were 0.480 ± 0.117 and 0.810 ± 0.114, respectively. The Young's moduli in Groups A-1 and A-2 were 576 ± 160 and 580 ± 150 kPa, respectively. In Groups A-1 and A-2, the normalized work to failure was 0.35 ± 0.14 and 1.11 ± 0.38 N. The specimens in Group B-1 withstood an average ultimate stress of 780 ± 280 kPa. The specimens in Group B-2 withstood an average ultimate stress of 405 ± 20 kPa. The average ultimate strains for Groups B-1 and B-2 were 0.319 ± 0.087 and 0.266 ± 0.019, respectively. The Young's moduli in Groups B-1 and B-2 were 4,030 ± 1360 and 2,290 ± 280 kPa, respectively. The normalized work to failure in Groups B-1 and B-2 was calculated as 0.22 ± 0.04 and 0.11 ± 0.02 N. Conclusions. Although adequately robust for reconstructive procedures, the acellular peripheral nerve had decreased tensile strength compared with fresh nerve either when tested whole or when transected and repaired.",
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N2 - Background. Acellular nerve has been used in experimental models as a peripheral nerve substitute. Our objective was to determine the difference in tensile strength between fresh and chemically treated acellularized peripheral nerve. Materials and methods. F344 rat sciatic nerves were either fresh or acellularized and tested either whole (Part A) or transected and repaired (Part B). For all constructs, the mean ultimate stress, mean ultimate strain, Young's modulus, and total mechanical work to fracture were calculated. Results. The average ultimate strains for Groups A-1 and A-2 were 0.480 ± 0.117 and 0.810 ± 0.114, respectively. The Young's moduli in Groups A-1 and A-2 were 576 ± 160 and 580 ± 150 kPa, respectively. In Groups A-1 and A-2, the normalized work to failure was 0.35 ± 0.14 and 1.11 ± 0.38 N. The specimens in Group B-1 withstood an average ultimate stress of 780 ± 280 kPa. The specimens in Group B-2 withstood an average ultimate stress of 405 ± 20 kPa. The average ultimate strains for Groups B-1 and B-2 were 0.319 ± 0.087 and 0.266 ± 0.019, respectively. The Young's moduli in Groups B-1 and B-2 were 4,030 ± 1360 and 2,290 ± 280 kPa, respectively. The normalized work to failure in Groups B-1 and B-2 was calculated as 0.22 ± 0.04 and 0.11 ± 0.02 N. Conclusions. Although adequately robust for reconstructive procedures, the acellular peripheral nerve had decreased tensile strength compared with fresh nerve either when tested whole or when transected and repaired.

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KW - Nerve regeneration

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KW - Tensile strength

KW - Tissue engineering

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