Mechanism of ATP-dependent promoter melting by transcription factor IIH

Tae Kyung Kim, Richard H. Ebright, Danny Reinberg

Research output: Contribution to journalArticle

201 Scopus citations

Abstract

We show that transcription factor IIH ERCC3 subunit; the DNA helicase responsible for adenosine triphosphate (ATP)-dependent promoter melting during transcription initiation, does not interact with the promoter region that undergoes melting but instead interacts with DNA downstream of this region. We show further that promoter melting does not change protein-DNA interactions upstream of the region that undergoes melting but does change interactions within and downstream of this region. Our results rule out the proposal that IIH functions in promoter melting through a conventional DNA- helicase mechanism. We propose that IIH functions as a molecular wrench: rotating downstream DNA relative to fixed upstream protein-DNA interactions, thereby generating torque on, and melting, the intervening DNA.

Original languageEnglish (US)
Pages (from-to)1418-1421
Number of pages4
JournalScience
Volume288
Issue number5470
DOIs
StatePublished - May 26 2000

ASJC Scopus subject areas

  • General

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