TY - JOUR
T1 - Mechanism of caveolin filament assembly
AU - Fernandez, Imma
AU - Ying, Yunshu
AU - Albanesi, Joseph
AU - Anderson, Richard G W
PY - 2002/8/20
Y1 - 2002/8/20
N2 - Caveolin-1 was the first protein identified that colocalizes with the ≈10-nm filaments found on the inside surface of caveolae membranes. We have used a combination of electron microscopy (EM), circular dichroism, and analytical ultracentrifugation to determine the structure of the oligomers that form when the first 101 aa of caveolin-1 (Cav1-101) are allowed to associate. We determined that amino acids 79-96 in this caveolin-1 fragment are arranged in an α-helix. Cav1-101 oligomers are ≈11 nm in diameter and contain seven molecules of Cav1-101. These subunits, in turn, are able to assemble into 50 nm long x 11 nm diameter filaments that closely match the morphology of the filaments in the caveolae filamentous coat. We propose that the heptameric subunit forms in part through lateral interactions between the α-helices of the seven Cav1-101 units. Caveolin-1, therefore, appears to be the structural molecule of the caveolae filamentous coat.
AB - Caveolin-1 was the first protein identified that colocalizes with the ≈10-nm filaments found on the inside surface of caveolae membranes. We have used a combination of electron microscopy (EM), circular dichroism, and analytical ultracentrifugation to determine the structure of the oligomers that form when the first 101 aa of caveolin-1 (Cav1-101) are allowed to associate. We determined that amino acids 79-96 in this caveolin-1 fragment are arranged in an α-helix. Cav1-101 oligomers are ≈11 nm in diameter and contain seven molecules of Cav1-101. These subunits, in turn, are able to assemble into 50 nm long x 11 nm diameter filaments that closely match the morphology of the filaments in the caveolae filamentous coat. We propose that the heptameric subunit forms in part through lateral interactions between the α-helices of the seven Cav1-101 units. Caveolin-1, therefore, appears to be the structural molecule of the caveolae filamentous coat.
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U2 - 10.1073/pnas.172196599
DO - 10.1073/pnas.172196599
M3 - Article
C2 - 12167674
AN - SCOPUS:0037143666
SN - 0027-8424
VL - 99
SP - 11193
EP - 11198
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -