@inproceedings{3c1ac0a2069d4eb69ad96446966c6786,
title = "Mechanism of PNA transport to the nuclear compartment",
abstract = "We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promoter in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPγS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.",
keywords = "Digitonin, GTPγS, Karyopherins, N-ethylmaleimide, NEM, Nuclear pore complex, Oligonucleotide, Peptide nucleic acid, Permeabilized cells, SK-BR-3 cells",
author = "Lenka Stankova and Ziemba, {Amy J.} and Zhilina, {Zhanna V.} and Ebbinghaus, {Scot W.}",
note = "Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2006",
month = oct,
doi = "10.1196/annals.1348.064",
language = "English (US)",
isbn = "1573315877",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "27--30",
booktitle = "Oligonucleotide Therapeutics",
}