Mechanism of PNA transport to the nuclear compartment

Lenka Stankova, Amy J. Ziemba, Zhanna V. Zhilina, Scot W. Ebbinghaus

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promoter in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPγS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.

Original languageEnglish (US)
Title of host publicationOligonucleotide Therapeutics
Subtitle of host publicationFirst Annual Meeting of the Oligonucleotide Therapeutics Society
PublisherBlackwell Publishing Inc.
Pages27-30
Number of pages4
ISBN (Print)1573315877, 9781573315876
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1082
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Digitonin
  • GTPγS
  • Karyopherins
  • N-ethylmaleimide
  • NEM
  • Nuclear pore complex
  • Oligonucleotide
  • Peptide nucleic acid
  • Permeabilized cells
  • SK-BR-3 cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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  • Cite this

    Stankova, L., Ziemba, A. J., Zhilina, Z. V., & Ebbinghaus, S. W. (2006). Mechanism of PNA transport to the nuclear compartment. In Oligonucleotide Therapeutics: First Annual Meeting of the Oligonucleotide Therapeutics Society (pp. 27-30). (Annals of the New York Academy of Sciences; Vol. 1082). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1348.064