Mechanism of regulation of renal ion transport by WNK kinases

Chou Long Huang, Sung Sen Yang, Shih Hua Lin

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Purpose of review This review summarizes recent advances in the understanding of the mechanism of regulation of renal ion transport by WNK kinases. Recent findings There are four mammalian WNK [with-no-lysine (K)] kinases: WNK1 -WNK4. Mutations of WNK1 and WNK4 in humans cause hypertension and hyperkalemia at least partly by altering renal sodium and potassium transport. WNK1 and WNK4 stimulate endocytosis of ROMK1 by recruiting an endocytic scaffold protein, intersectin. The recruitment is independent of the kinase activity and occurs between the PXXP motif of WNKs and the SH3 domain of intersectin. Regulation of cation-chloride-coupled cotransporters, Na+-K +-2CP cotransporter (NKCC) 1 and NKCC2 [and the Na-Cl cotransporter (NCC), under some conditions] by WNKs requires kinase activity. WNK1 and WNK4 bind with and phosphorylate two Ste20-related protein kinases, OSR1 and SPAK, which in turn bind with and phosphorylate NKCCs and NCC to increase their activity. Binding of OSR1/SPAK to upstream activators (WNKs) and downstream substrates (NKCCs and NCC) are both mediated by a docking site in the C-terminus of OSR1/SPAK and RFX[V/I] motifs present in WNKs or in NKCCs and NCC. WNKs regulate ion transport via both catalytic and noncatalytic mechanisms. We discuss hypotheses that WNKs, contrasting with aldosterone, play important roles in dissociating sodium reabsorption from potassium secretion.

Original languageEnglish (US)
Pages (from-to)519-525
Number of pages7
JournalCurrent Opinion in Nephrology and Hypertension
Volume17
Issue number5
DOIs
StatePublished - Sep 2008

Fingerprint

Member 3 Solute Carrier Family 12
Ion Transport
Phosphotransferases
Kidney
Member 1 Solute Carrier Family 12
Potassium
Sodium
Hyperkalemia
src Homology Domains
Endocytosis
Aldosterone
Protein Kinases
Lysine
Cations
Chlorides
Hypertension
Mutation
Proteins

Keywords

  • Aldosterone
  • Gordon's syndrome
  • PHA2
  • Pseudohypoaldosteronism type 2
  • WNK1
  • WNK3
  • WNK4

ASJC Scopus subject areas

  • Nephrology
  • Internal Medicine

Cite this

Mechanism of regulation of renal ion transport by WNK kinases. / Huang, Chou Long; Yang, Sung Sen; Lin, Shih Hua.

In: Current Opinion in Nephrology and Hypertension, Vol. 17, No. 5, 09.2008, p. 519-525.

Research output: Contribution to journalArticle

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