Mechanisms of p185HER2 expression in human non-small cell lung cancer cell lines.

J. A. Kern, R. A. Robinson, A. Gazdar, L. Torney, D. B. Weiner

Research output: Contribution to journalArticle

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Abstract

To identify mechanisms that allow p185HER2 expression in lung cancer, we performed Western, Southern, and Northern blot analyses of 14 cell lines derived from human non-small cell lung carcinomas and one cell line derived from a human mesothelioma. Human bronchiole epithelial cells and rat type II pneumocytes were found to express p185HER2 at low to undetectable levels by Western blot technique. In contrast, 13 lung cancer cell lines expressed p185HER2, and eight of these 13 expressed p185HER2 at levels at least 2-fold higher than that found in normal bronchiole epithelial cells or type II pneumocytes. Genomic Southern analysis showed that amplification of the HER2 gene was present in only one of the eight cell lines that expressed p185HER2 at these higher levels. Increased levels of steady-state HER2 mRNA occurred in the remaining seven cell lines. We conclude that in human non-small cell lung carcinoma cell lines the most common mechanism resulting in increased p185HER2 expression is due to mechanisms that increase HER2 mRNA levels, with HER2 gene amplification occurring less commonly.

Original languageEnglish (US)
Pages (from-to)359-363
Number of pages5
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume6
Issue number4
StatePublished - Apr 1992

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Non-Small Cell Lung Carcinoma
Cells
Cell Line
erbB-2 Genes
Alveolar Epithelial Cells
Bronchioles
Lung Neoplasms
Western Blotting
Epithelial Cells
Messenger RNA
Gene Amplification
Mesothelioma
Southern Blotting
Northern Blotting
Amplification
Rats
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Kern, J. A., Robinson, R. A., Gazdar, A., Torney, L., & Weiner, D. B. (1992). Mechanisms of p185HER2 expression in human non-small cell lung cancer cell lines. American Journal of Respiratory Cell and Molecular Biology, 6(4), 359-363.

Mechanisms of p185HER2 expression in human non-small cell lung cancer cell lines. / Kern, J. A.; Robinson, R. A.; Gazdar, A.; Torney, L.; Weiner, D. B.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 6, No. 4, 04.1992, p. 359-363.

Research output: Contribution to journalArticle

Kern, JA, Robinson, RA, Gazdar, A, Torney, L & Weiner, DB 1992, 'Mechanisms of p185HER2 expression in human non-small cell lung cancer cell lines.', American Journal of Respiratory Cell and Molecular Biology, vol. 6, no. 4, pp. 359-363.
Kern, J. A. ; Robinson, R. A. ; Gazdar, A. ; Torney, L. ; Weiner, D. B. / Mechanisms of p185HER2 expression in human non-small cell lung cancer cell lines. In: American Journal of Respiratory Cell and Molecular Biology. 1992 ; Vol. 6, No. 4. pp. 359-363.
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