Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis

Diane G. Edmondson, Gary E. Lyons, James F. Martin, Eric N. Olson

Research output: Contribution to journalArticle

420 Citations (Scopus)

Abstract

Members of the MEF2 family of transcription factors bind a conserved A/T-rich sequence in the control regions of many skeletal and cardiac muscle genes. To begin to assess the roles of the different Mef2 genes in the control of muscle gene expression in vivo, we analyzed by in situ hybridization the expression patterns of the Mef2a, Mef2c and Mef2d genes during mouse embryogenesis. We first detected MEF2C expression at day 7.5 postcoitum (p.c.) in cells of the cardiac mesoderm that give rise to the primitive heart tube, making MEF2C one of the earliest markers for the cardiac muscle lineage yet described. By day 8.5, MEF2A, MEF2C and MEF2D mRNAs are all detected in the myocardium. By day 9.0, MEF2C is expressed in rostral myotomes, where its expression lags by about a day behind that of myf5 and several hours behind that of myogenin. MEF2A and MEF2D are expressed at a lower level than MEF2C in the myotome at day 9.5 and are detected in more embryonic tissues than MEF2C. Expression of each of the MEF2 transcripts is observed in muscle-forming regions within the limbs at day 11.5 p.c. and within muscle fibers throughout the embryo at later developmental stages. The expression of MEF2C in the somites and fetal muscle is distinct from that of MEF2A, MEF2D and the myogenic bHLH regulatory genes, suggesting that it may represent a distinct myogenic cell type. Neural crest cells also express high levels of MEF2 mRNAs between days 8.5 and 10.5 of gestation. After day 12.5 p.c., MEF2 transcripts are detected at high levels in specific regions of the brain and ultimately in a wide range of tissues. The distinct patterns of expression of the different Mef2 genes during mouse embryogenesis suggest that these genes respond to unique developmental cues and support the notion that their products play roles in the regulation of muscle-specific transcription during establishment of the cardiac and skeletal muscle lineages.

Original languageEnglish (US)
Pages (from-to)1251-1263
Number of pages13
JournalDevelopment
Volume120
Issue number5
StatePublished - May 1994

Fingerprint

Embryonic Development
Myocardium
Skeletal Muscle
Gene Expression
Muscles
Genes
MEF2 Transcription Factors
Myogenin
Somites
Messenger RNA
Neural Crest
Mesoderm
Regulator Genes
In Situ Hybridization
Cues
Embryonic Structures
Extremities
Pregnancy
Brain

Keywords

  • MEF2
  • Mouse
  • Muscle gene expression
  • Myogenesis

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis. / Edmondson, Diane G.; Lyons, Gary E.; Martin, James F.; Olson, Eric N.

In: Development, Vol. 120, No. 5, 05.1994, p. 1251-1263.

Research output: Contribution to journalArticle

Edmondson, DG, Lyons, GE, Martin, JF & Olson, EN 1994, 'Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis', Development, vol. 120, no. 5, pp. 1251-1263.
Edmondson, Diane G. ; Lyons, Gary E. ; Martin, James F. ; Olson, Eric N. / Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis. In: Development. 1994 ; Vol. 120, No. 5. pp. 1251-1263.
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