MEF2B is a potent transactivator expressed in early myogenic lineages

Jeffery D. Molkentin, Anthony B. Firulli, Brian L. Black, James F. Martin, Carolyn M. Hustad, Neal Copeland, Nancy Jenkins, Gary Lyons, Eric N. Olson

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

There are four members of the myocyte enhancer binding factor 2 (MEF2) family of transcription factors, MEF2A, -B, -C, and -D, that have homology within an amino-terminal MADS box and an adjacent MEF2 domain that together mediate dimerization and DNA binding. MEF2A, -C, and -D have previously been shown to bind an A/T-rich DNA sequence in the control regions of numerous muscle-specific genes, whereas MEF2B was reported to be unable to bind this sequence unless the carboxyl terminus was deleted. To further define the functions of MEF2B, we analyzed its DNA binding and transcriptional activities. In contrast to previous studies, our results show that MEF2B binds the same DNA sequence as other members of the MEF2 family and acts as a strong transactivator through that sequence. Transcriptional activation by MEF2B is dependent on the carboxyl terminus, which contains two conserved sequence motifs found in all vertebrate MEF2 factors. During mouse embryogenesis, MEF2B transcripts are expressed in the developing cardiac and skeletal muscle lineages in a temporospatial pattern distinct from but overlapping with those of the other Mef2 genes. The mouse Mef2b gene maps to chromosome 8 and is unlinked to other Mef2 genes; its intron-exon organization is similar to that of the other vertebrate Mef2 genes and the single Drosophila Mef2 gene, consistent with the notion that these different Mef2 genes evolved from a common ancestral gene.

Original languageEnglish (US)
Pages (from-to)3814-3824
Number of pages11
JournalMolecular and Cellular Biology
Volume16
Issue number7
StatePublished - 1996

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Trans-Activators
MEF2 Transcription Factors
Genes
Vertebrates
Chromosomes, Human, Pair 8
Conserved Sequence
DNA
Dimerization
Introns
Transcriptional Activation
Drosophila
Embryonic Development
Exons
Myocardium
Skeletal Muscle
Muscles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Molkentin, J. D., Firulli, A. B., Black, B. L., Martin, J. F., Hustad, C. M., Copeland, N., ... Olson, E. N. (1996). MEF2B is a potent transactivator expressed in early myogenic lineages. Molecular and Cellular Biology, 16(7), 3814-3824.

MEF2B is a potent transactivator expressed in early myogenic lineages. / Molkentin, Jeffery D.; Firulli, Anthony B.; Black, Brian L.; Martin, James F.; Hustad, Carolyn M.; Copeland, Neal; Jenkins, Nancy; Lyons, Gary; Olson, Eric N.

In: Molecular and Cellular Biology, Vol. 16, No. 7, 1996, p. 3814-3824.

Research output: Contribution to journalArticle

Molkentin, JD, Firulli, AB, Black, BL, Martin, JF, Hustad, CM, Copeland, N, Jenkins, N, Lyons, G & Olson, EN 1996, 'MEF2B is a potent transactivator expressed in early myogenic lineages', Molecular and Cellular Biology, vol. 16, no. 7, pp. 3814-3824.
Molkentin JD, Firulli AB, Black BL, Martin JF, Hustad CM, Copeland N et al. MEF2B is a potent transactivator expressed in early myogenic lineages. Molecular and Cellular Biology. 1996;16(7):3814-3824.
Molkentin, Jeffery D. ; Firulli, Anthony B. ; Black, Brian L. ; Martin, James F. ; Hustad, Carolyn M. ; Copeland, Neal ; Jenkins, Nancy ; Lyons, Gary ; Olson, Eric N. / MEF2B is a potent transactivator expressed in early myogenic lineages. In: Molecular and Cellular Biology. 1996 ; Vol. 16, No. 7. pp. 3814-3824.
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