Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling

Yun Li, Jean Z. Maines, Ömür Y. Tastan, Dennis M. McKearin, Michael Buszczak

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In the Drosophila ovary, bone morphogenetic protein (BMP) ligands maintain germline stem cells (GSCs) in an undifferentiated state. The activation of the BMP pathway within GSCs results in the transcriptional repression of the differentiation factor bag of marbles (bam). The Nanos-Pumilio translational repressor complex and the miRNA pathway also help to promote GSC selfrenewal. How the activities of different transcriptional and translational regulators are coordinated to keep the GSC in an undifferentiated state remains uncertain. Data presented here show that Mei-P26 cell-autonomously regulates GSC maintenance in addition to its previously described role of promoting germline cyst development. Within undifferentiated germ cells, Mei-P26 associates with miRNA pathway components and represses the translation of a shared target mRNA, suggesting that Mei-P26 can enhance miRNA-mediated silencing in specific contexts. In addition, disruption of mei-P26 compromises BMP signaling, resulting in the inappropriate expression of bam in germ cells immediately adjacent to the cap cell niche. Loss of mei-P26 results in premature translation of the BMP antagonist Brat in germline stem cells. These data suggest that Mei-P26 has distinct functions in the ovary and participates in regulating the fates of both GSCs and their differentiating daughters.

Original languageEnglish (US)
Pages (from-to)1547-1556
Number of pages10
JournalDevelopment
Volume139
Issue number9
DOIs
StatePublished - May 1 2012

Fingerprint

Bone Morphogenetic Proteins
Stem Cells
Maintenance
MicroRNAs
Calcium Carbonate
Germ Cells
Ovary
Oogonial Stem Cells
Drosophila
Cysts
Ligands
Messenger RNA

Keywords

  • Drosophila
  • Germline
  • Repression
  • Stem cells
  • Translation
  • Tumor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling. / Li, Yun; Maines, Jean Z.; Tastan, Ömür Y.; McKearin, Dennis M.; Buszczak, Michael.

In: Development, Vol. 139, No. 9, 01.05.2012, p. 1547-1556.

Research output: Contribution to journalArticle

Li, Yun ; Maines, Jean Z. ; Tastan, Ömür Y. ; McKearin, Dennis M. ; Buszczak, Michael. / Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling. In: Development. 2012 ; Vol. 139, No. 9. pp. 1547-1556.
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