Meiotic expression of the cyclin H/Cdk7 complex in male germ cells of the mouse

J. M. Kim, J. T. McGaughy, R. K. Bogle, S. E. Ravnik

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cell division requires that cyclin-dependent kinases (Cdks) be activated by phosphorylation. In mitotic cells, this is accomplished by the Cdk-activating-kinase (CAK), which is a complex of cyclin H and Cdk7. There are currently no data on the role of CAK in meiotic cells. Previously, we have shown that cyclin A1 is meiosis-specific and forms an active kinase with Cdk2. Because cyclin A1 is required for meiosis, and its associated kinase must be phosphorylated (activated), we propose that cydin H/Cdk7 function to activate cyclin A1/Cdk2 in meiotic cells. Here, we show that cyclin H and Cdk7 are present during meiosis. Using reverse transcription-polymerase chain reaction and in situ hybridization, we show that the mRNAs encoding cyclin H and Cdk7 are abundant in spermatocytes. Immunohistochemistry localized cyclin H and Cdk7 to the nucleus of spermatocytes in stages IV to XII of the spermatogenic cycle, overlapping the same stages that express cyclin A1-associated kinases. Finally, immunoprecipitation and histone H1-kinase assays of cyclin H and Cdk7 from testicular extracts show that these proteins interact to form an active kinase. We conclude that cyclin H/Cdk7 complexes are present and during meiosis, form active complexes in testicular cells and are strong candidates for the activating kinase for cyclin A1-associated kinase.

Original languageEnglish (US)
Pages (from-to)1400-1408
Number of pages9
JournalBiology of Reproduction
Volume64
Issue number5
StatePublished - 2001

Fingerprint

Cyclin H
Cyclin A1
Germ Cells
Phosphotransferases
Meiosis
Spermatocytes
Cyclin-Dependent Kinases
Immunoprecipitation
Cell Division
Reverse Transcription
In Situ Hybridization
Immunohistochemistry
Phosphorylation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Developmental biology
  • Gametogenesis
  • Meiosis
  • Spermatogenesis
  • Testes

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Meiotic expression of the cyclin H/Cdk7 complex in male germ cells of the mouse. / Kim, J. M.; McGaughy, J. T.; Bogle, R. K.; Ravnik, S. E.

In: Biology of Reproduction, Vol. 64, No. 5, 2001, p. 1400-1408.

Research output: Contribution to journalArticle

Kim, J. M. ; McGaughy, J. T. ; Bogle, R. K. ; Ravnik, S. E. / Meiotic expression of the cyclin H/Cdk7 complex in male germ cells of the mouse. In: Biology of Reproduction. 2001 ; Vol. 64, No. 5. pp. 1400-1408.
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