Meiotic prophase requires proteolysis of m phase regulators mediated by the meiosis-specific APC/CAma1

Elwy Okaz, Orlando Argüello-Miranda, Aliona Bogdanova, P. K. Vinod, Jesse J. Lipp, Zuzana Markova, Ievgeniia Zagoriy, Bela Novak, Wolfgang Zachariae

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.

Original languageEnglish (US)
Pages (from-to)603-618
Number of pages16
JournalCell
Volume151
Issue number3
DOIs
StatePublished - Oct 26 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Okaz, E., Argüello-Miranda, O., Bogdanova, A., Vinod, P. K., Lipp, J. J., Markova, Z., Zagoriy, I., Novak, B., & Zachariae, W. (2012). Meiotic prophase requires proteolysis of m phase regulators mediated by the meiosis-specific APC/CAma1. Cell, 151(3), 603-618. https://doi.org/10.1016/j.cell.2012.08.044