MEKK1 binds Raf-1 and the ERK2 cascade components

Mahesh Karandikar, Shuichan Xu, Melanie H. Cobb

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Mitogen-activated protein (MAP) kinase caseades are involed in transmitting signals that are generated at the cell surface into the cytosol and nucleus and consist of three sequentially acting enzymes: a MAP kinase, an upstream MAP/extracellular signal-regulated protein kinase (ERK) kinase (MEK), and a MEK kinase (MEKK). Protein-protein interactions within these cascades provide a mechanism to control the localization and function of the protiens. MEKK1 is implicated in activation of the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and ERK1/2 MAP kinase pathways. We showed previously that MEKK1 binds directly to JNK/SAPK. In this study we demonstrate that endogenous MEKK1 binds to endogenous ERK2, MEK1, and another MEKK level kinase, Raf-1, suggesting that it can assemble all three proteins of the ERK2 MAP kinase module.

Original languageEnglish (US)
Pages (from-to)40120-40127
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number51
DOIs
StatePublished - Dec 22 2000

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Mitogen-Activated Protein Kinases
MAP Kinase Kinase Kinases
Protein Kinases
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 11
Proteins
Proto-Oncogene Proteins c-raf
Mitogen-Activated Protein Kinase 1
Extracellular Signal-Regulated MAP Kinases
Heat-Shock Proteins
Mitogens
Cytosol
Chemical activation
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

MEKK1 binds Raf-1 and the ERK2 cascade components. / Karandikar, Mahesh; Xu, Shuichan; Cobb, Melanie H.

In: Journal of Biological Chemistry, Vol. 275, No. 51, 22.12.2000, p. 40120-40127.

Research output: Contribution to journalArticle

Karandikar, Mahesh ; Xu, Shuichan ; Cobb, Melanie H. / MEKK1 binds Raf-1 and the ERK2 cascade components. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 51. pp. 40120-40127.
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