Membrane-bound and soluble porcine CD83 functions antithetically in T cell activation and dendritic cell differentiation in vitro

Shanshan Huo, Jianlou Zhang, Shuang Liang, Fengyang Wu, Yuzhu Zuo, Dan Cui, Yonghong Zhang, Zhenyu Zhong, Fei Zhong

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Emerging evidence suggests that CD83, a dendritic cells (DCs) maturation marker in humans and mice, may prossess immunomodulatory capacities. Although porcine CD83 shares ∼75% sequence homology with its human counterpart, whether it functions as an immunoregulatory molecule remains unknown. To investigate porcine CD83 function, we deleted it in porcine DCs by RNA intereference. Results show that membrane-bound CD83 (mCD83) promotes DC-mediated T cell proliferation and cytokine production, thus confirming its immunoregulatory capacity. Intriguingly, porcine soluble CD83 (sCD83) treatment instead led to inhibition of DC-mediated T cell activation. Moreover, porcine sCD83 also inhibited differentiation of prepheral blood mononuclear cells (PBMCs) into DCs. These results collectively indicate that in addition to being a DC maturation maker, both membrane bound and souble porcine CD83 serve as immunoregulatory molecules with opposite effects on DC-mediated T cell activation and DC differentiation.

Original languageEnglish (US)
Article number103398
JournalDevelopmental and Comparative Immunology
Volume99
DOIs
StatePublished - Oct 2019

Keywords

  • Dendritic cells
  • Functional identification
  • Porcine CD83
  • T cell activation

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Membrane-bound and soluble porcine CD83 functions antithetically in T cell activation and dendritic cell differentiation in vitro'. Together they form a unique fingerprint.

Cite this