Membrane-bound progesterone receptor expression in human aortic endothelial cells

Brenda H. Welter, Elizabeth L. Hansen, Karla J. Saner, Yangzhan Wei, Thomas M. Price

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Observational studies demonstrate that estradiol and progesterone affect vasoreactivity. In animal studies, progesterone treatment causes immediate relaxation of precontracted arteries with inhibition of calcium influx in vascular endothelial and smooth muscle cells, suggesting a non-genomic mechanism of action. In this study we investigated the presence of novel membrane-bound progesterone receptors in human aortic endothelial cells and correlated the expression with cell-cycle stage. Western blotting analysis with an antibody directed to the hormone-binding domain of the classic progesterone receptors shows predominant bands at 100 and 60 kD, whereas analysis with an antibody to the DNA-binding region shows only the 100-kD band. In contrast, classic nuclear progesterone receptors B and A are identified at 116 and 94 kD in similarly processed T47D cells. Both novel bands localize to the membrane fraction after differential centrifugation. Plasma membrane-bound progesterone receptor was further shown with immunofluorescent antibody and ligand-binding studies in a small percentage of human aortic endothelial cells. Fluorescent activated cell sorting demonstrated that approximately 8% of the human aortic endothelial cells expressed a plasma membrane progesterone receptor and that a greater percentage of the expressing cells were in the G2/M-phase of the cell cycle. Treatment with progesterone conjugated to BSA did not show any significant cell-cycle changes. Plasma membrane-bound progesterone receptor in vascular endothelial cells may regulate the non-genomic actions of progesterone, and expression of the receptor appears to vary with cell cycle stage.

Original languageEnglish (US)
Pages (from-to)1049-1055
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number8
StatePublished - Aug 1 2003

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Progesterone Receptors
Endothelial Cells
Membranes
Cell Cycle
Progesterone
Cell Membrane
Antibodies
G2 Phase
Cytoplasmic and Nuclear Receptors
Centrifugation
Vascular Smooth Muscle
Cell Division
Smooth Muscle Myocytes
Observational Studies
Estradiol
Arteries
Western Blotting
Hormones
Ligands
Calcium

Keywords

  • Cell cycle
  • Human aortic endothelial cells
  • Membrane-bound
  • Progesterone receptor

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Membrane-bound progesterone receptor expression in human aortic endothelial cells. / Welter, Brenda H.; Hansen, Elizabeth L.; Saner, Karla J.; Wei, Yangzhan; Price, Thomas M.

In: Journal of Histochemistry and Cytochemistry, Vol. 51, No. 8, 01.08.2003, p. 1049-1055.

Research output: Contribution to journalArticle

Welter, Brenda H. ; Hansen, Elizabeth L. ; Saner, Karla J. ; Wei, Yangzhan ; Price, Thomas M. / Membrane-bound progesterone receptor expression in human aortic endothelial cells. In: Journal of Histochemistry and Cytochemistry. 2003 ; Vol. 51, No. 8. pp. 1049-1055.
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