@article{08390087eccd46058103c71a6540b94a,
title = "Membrane curvature response in autophagy",
abstract = "Membrane trafficking is key for signal transduction, cargo transportation, and in the case of autophagy, delivering cytoplasmic substrates to the lysosome for degradation. Autophagy requires the formation of a unique double membrane vesicle, the autophagosome. However, the mechanism by which the autophagosome forms is unknown. Our recent study focused on the role of Barkor/Atg14(L) in targeting the autophagy-specific class III phosphatidylinositol-3-kinase (PtdIns3KC3) complex to the early autophagosome has implicated this complex in autophagosome formation. This study found that the BATS domain of Barkor targets the PtdIns3KC3 complex to early autophagic structures and senses highly curved membranes enriched in phosphatidylinositol-3-phosphate (PtdIns(3)P). Consequently, this study uncovered an exciting new role for the PtdIns3KC3 complex as a potential inducer of autophagosome formation.",
keywords = "ALPS, Amphipathic alpha helix, Atg14(L), Autophagosome, Autophagy, BATS, Barkor, Membrane curvature, PtdIns(3)P, PtdIns3KC3",
author = "Livia Wilz and Weiliang Fan and Qing Zhong",
note = "Funding Information: PtdIns3KC3 complexily protein. Upon intDo not, Bif-1 regulates curvature theraction with the PtdIns(3)Pdat couproducild etiosvennanttualdmrly bemie sbrabta-ne uformatchemictional me. To aech.ccomplish this, we must anism of autophagosome PtdIns3KC3 kinase activity; elimina-bilized by the additional recruitment of better understand the interplay between tion of Bif-1 expression compromises PtdIns(3)P binding proteins (DFCP1 the PtdIns3K3C complex and the early autophagosome formation. These results and WIPI1/2) or coat-forming pro-autophagosome membrane and also deci-imply that Bif-1 could function like other teins (Atg12-Atg5-Atg16), allowing the pher how other early autophagic machin-N-BAR domain proteins and induce and omegasome to develop into a mature ery are recruited to the membrane and stabilize autophagosome membrane cur-autophagosome. interact with each other to induce auto-vature. However, since Bif-1 associates This study also proposes a critical phagosome formation. with the PtdIns3KC3 complex through role of PtdIns(3)P in membrane cur- UVRAG, which is implicated in later vature sensing and stabilization dur- autophagic steps such as autophagosome ing autophagosome formation, which is Q.Z. is supported by a New Investigator maturation, it is an unlikely major com-supported by several lines of evidence. Award for Aging from the Ellison Medical ponent of autophagosome formation. PtdIns(3)P production is essential for the Foundation, Hellman Family Fund and Consequently, the discovery of the biogenesis of autophagosomes. Depletion NIH RO1(CA133228). W.F. is funded by amphipathic alpha helix in the BATS of PtdIns(3)P decreases membrane Chinese Scholarship Council.",
year = "2011",
month = oct,
doi = "10.4161/auto.7.10.16738",
language = "English (US)",
volume = "7",
pages = "1249--1250",
journal = "Autophagy",
issn = "1554-8627",
publisher = "Landes Bioscience",
number = "10",
}