TY - JOUR
T1 - Membrane ecto-ATPase on epidermal Langerhans cells
AU - Girolomoni, Giampiero
AU - Santantonio, Maria Lucia
AU - Bergstresser, Paul R.
AU - Cruz, Ponciano D
AU - Giannetti, Alberto
PY - 1992/11
Y1 - 1992/11
N2 - Epidermal Langerhans cells (LC) possess a plasma membrane Mg++- or Ca++-dependent ecto-ATPase (mATPase), the function of which is not known. On the other hand, extracellular ATP (ATP°) has been shown to cause a rapid increase in cell-membrane permeability for selected cells, thus inducing the entry of exogenous substances, leading eventually to cell death. ATP°has also been shown to be a mediator of T cell - mediated cytotoxicity. We have observed human LC to be resistant to the permeabilizing effects of ATP°when compared to cultured human keratinocytes and J774 macrophages. In addition, our preliminary evidence in vitro suggests that mATPase on LC may provide protection against the lytic effects of ATP°. Finally, we propose that this latter mechanism may also operate in vivo.
AB - Epidermal Langerhans cells (LC) possess a plasma membrane Mg++- or Ca++-dependent ecto-ATPase (mATPase), the function of which is not known. On the other hand, extracellular ATP (ATP°) has been shown to cause a rapid increase in cell-membrane permeability for selected cells, thus inducing the entry of exogenous substances, leading eventually to cell death. ATP°has also been shown to be a mediator of T cell - mediated cytotoxicity. We have observed human LC to be resistant to the permeabilizing effects of ATP°when compared to cultured human keratinocytes and J774 macrophages. In addition, our preliminary evidence in vitro suggests that mATPase on LC may provide protection against the lytic effects of ATP°. Finally, we propose that this latter mechanism may also operate in vivo.
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M3 - Article
SN - 0022-202X
VL - 99
SP - 12
EP - 14
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -