Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs

Dayoung Oh, Jung Ah Song, Jung Sun Moon, Mi Jin Moon, Jae Il Kim, Kyungjin Kim, Hyuk Bang Kwon, Jae Young Seong

Research output: Contribution to journalArticle

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Abstract

Recently, we demonstrated that the mammalian type-l GnRH receptor (GnRHR) has a high preference for the phospholipase C/protein kinase C (PLC/PKC)-linked signaling pathway, whereas non-mammalian bullfrog (bf) GnRHRs couple to both adenylate cyclase/protein kinase A (AC/PKA)- and PLC/PKC-linked signaling pathways. In the present study, using AC/PKA-specific reporter (cAMP-responsive element-luciferase) and PLC/ PKC-specific reporter (serum-responsive element-luciferase) systems, we attempted to identify the motif responsible for this difference. A deletion of the intracellular carboxyl-terminal tail (C tail) of bfGnRHR-1 remarkably decreased its ability to induce the AC/PKA-linked signaling pathway. Further dissection of the C tail indicated that an HFRK motif in the membrane-proximal sequence of bfGnRHR-1 C tail is a minimal requirement for the AC/PKA-linked signaling pathway as the addition of this motif to rat GnRHR or deletion of it from bfGnRHR-1 significantly affected the ability to induce the AC/PKA-linked signaling pathway. Deletion or addition of the HFRK motif, however, did not critically influence the PLC/PKC-linked signaling pathway. These results indicate that the HFRK motif in the membrane-proximal region confers the differential signal transduction pathways between mammalian and nonmammalian GnRHRs.

Original languageEnglish (US)
Pages (from-to)722-731
Number of pages10
JournalMolecular Endocrinology
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2005

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LHRH Receptors
Cyclic AMP-Dependent Protein Kinases
Adenylyl Cyclases
Signal Transduction
Protein Kinase C
Type C Phospholipases
Membranes
Tail
Luciferases
Rana catesbeiana
Dissection
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs. / Oh, Dayoung; Song, Jung Ah; Moon, Jung Sun; Moon, Mi Jin; Kim, Jae Il; Kim, Kyungjin; Kwon, Hyuk Bang; Seong, Jae Young.

In: Molecular Endocrinology, Vol. 19, No. 3, 01.03.2005, p. 722-731.

Research output: Contribution to journalArticle

Oh, Dayoung ; Song, Jung Ah ; Moon, Jung Sun ; Moon, Mi Jin ; Kim, Jae Il ; Kim, Kyungjin ; Kwon, Hyuk Bang ; Seong, Jae Young. / Membrane-proximal region of the carboxyl terminus of the gonadotropin-releasing hormone receptor (GnRHR) confers differential signal transduction between mammalian and nonmammalian GnRHRs. In: Molecular Endocrinology. 2005 ; Vol. 19, No. 3. pp. 722-731.
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abstract = "Recently, we demonstrated that the mammalian type-l GnRH receptor (GnRHR) has a high preference for the phospholipase C/protein kinase C (PLC/PKC)-linked signaling pathway, whereas non-mammalian bullfrog (bf) GnRHRs couple to both adenylate cyclase/protein kinase A (AC/PKA)- and PLC/PKC-linked signaling pathways. In the present study, using AC/PKA-specific reporter (cAMP-responsive element-luciferase) and PLC/ PKC-specific reporter (serum-responsive element-luciferase) systems, we attempted to identify the motif responsible for this difference. A deletion of the intracellular carboxyl-terminal tail (C tail) of bfGnRHR-1 remarkably decreased its ability to induce the AC/PKA-linked signaling pathway. Further dissection of the C tail indicated that an HFRK motif in the membrane-proximal sequence of bfGnRHR-1 C tail is a minimal requirement for the AC/PKA-linked signaling pathway as the addition of this motif to rat GnRHR or deletion of it from bfGnRHR-1 significantly affected the ability to induce the AC/PKA-linked signaling pathway. Deletion or addition of the HFRK motif, however, did not critically influence the PLC/PKC-linked signaling pathway. These results indicate that the HFRK motif in the membrane-proximal region confers the differential signal transduction pathways between mammalian and nonmammalian GnRHRs.",
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