MEMS-based handheld fourier domain doppler optical coherence tomography for intraoperative microvascular anastomosis imaging

Yong Huang, Georg J. Furtmüller, Dedi Tong, Shan Zhu, W. P.Andrew Lee, Gerald Brandacher, Jin U. Kang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: To demonstrate the feasibility of a miniature handheld optical coherence tomography (OCT) imager for real time intraoperative vascular patency evaluation in the setting of super-microsurgical vessel anastomosis.

Methods: A novel handheld imager Fourier domain Doppler optical coherence tomography based on a 1.3-mm central wavelength swept source for extravascular imaging was developed. The imager was minimized through the adoption of a 2.4-μm diameter microelectromechanical systems (MEMS) scanning mirror, additionally a 12.7-mm diameter lens system was designed and combined with the MEMS mirror to achieve a small form factor that optimize functionality as a handheld extravascular OCT imager. To evaluate in-vivo applicability, supermicrosurgical vessel anastomosis was performed in a mouse femoral vessel cut and repair model employing conventional interrupted suture technique as well as a novel non-suture cuff technique. Vascular anastomosis patency after clinically successful repair was evaluated using the novel handheld OCT imager.

Results: With an adjustable lateral image field of view up to 1.5 mm by 1.5 mm, high-resolution simultaneous structural and flow imaging of the blood vessels were successfully acquired for BALB/C mouse after orthotopic hind limb transplantation using a non-suture cuff technique and BALB/C mouse after femoral artery anastomosis using a suture technique. We experimentally quantify the axial and lateral resolution of the OCT to be 12.6 μm in air and 17.5 μm respectively. The OCT has a sensitivity of 84 dB and sensitivity roll-off of 5.7 dB/mm over an imaging range of 5 mm. Imaging with a frame rate of 36 Hz for an image size of 1000(lateral)×512(axial) pixels using a 50,000 A-lines per second swept source was achieved. Quantitative vessel lumen patency, lumen narrowing and thrombosis analysis were performed based on acquired structure and Doppler images.

Conclusions: A miniature handheld OCT imager that can be used for intraoperative evaluation of microvascular anastomosis was successfully demonstrated.

Original languageEnglish (US)
Article numbere114215
JournalPloS one
Volume9
Issue number12
DOIs
StatePublished - Dec 4 2014
Externally publishedYes

Fingerprint

Optical tomography
tomography
Optical Coherence Tomography
MEMS
Image sensors
image analysis
Imaging techniques
Vascular Patency
suture techniques
Inbred BALB C Mouse
blood vessels
Suture Techniques
thighs
mice
Mirrors
Repair
thrombosis
Blood vessels
Femoral Artery
Thigh

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

MEMS-based handheld fourier domain doppler optical coherence tomography for intraoperative microvascular anastomosis imaging. / Huang, Yong; Furtmüller, Georg J.; Tong, Dedi; Zhu, Shan; Lee, W. P.Andrew; Brandacher, Gerald; Kang, Jin U.

In: PloS one, Vol. 9, No. 12, e114215, 04.12.2014.

Research output: Contribution to journalArticle

Huang, Yong ; Furtmüller, Georg J. ; Tong, Dedi ; Zhu, Shan ; Lee, W. P.Andrew ; Brandacher, Gerald ; Kang, Jin U. / MEMS-based handheld fourier domain doppler optical coherence tomography for intraoperative microvascular anastomosis imaging. In: PloS one. 2014 ; Vol. 9, No. 12.
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abstract = "Purpose: To demonstrate the feasibility of a miniature handheld optical coherence tomography (OCT) imager for real time intraoperative vascular patency evaluation in the setting of super-microsurgical vessel anastomosis.Methods: A novel handheld imager Fourier domain Doppler optical coherence tomography based on a 1.3-mm central wavelength swept source for extravascular imaging was developed. The imager was minimized through the adoption of a 2.4-μm diameter microelectromechanical systems (MEMS) scanning mirror, additionally a 12.7-mm diameter lens system was designed and combined with the MEMS mirror to achieve a small form factor that optimize functionality as a handheld extravascular OCT imager. To evaluate in-vivo applicability, supermicrosurgical vessel anastomosis was performed in a mouse femoral vessel cut and repair model employing conventional interrupted suture technique as well as a novel non-suture cuff technique. Vascular anastomosis patency after clinically successful repair was evaluated using the novel handheld OCT imager.Results: With an adjustable lateral image field of view up to 1.5 mm by 1.5 mm, high-resolution simultaneous structural and flow imaging of the blood vessels were successfully acquired for BALB/C mouse after orthotopic hind limb transplantation using a non-suture cuff technique and BALB/C mouse after femoral artery anastomosis using a suture technique. We experimentally quantify the axial and lateral resolution of the OCT to be 12.6 μm in air and 17.5 μm respectively. The OCT has a sensitivity of 84 dB and sensitivity roll-off of 5.7 dB/mm over an imaging range of 5 mm. Imaging with a frame rate of 36 Hz for an image size of 1000(lateral)×512(axial) pixels using a 50,000 A-lines per second swept source was achieved. Quantitative vessel lumen patency, lumen narrowing and thrombosis analysis were performed based on acquired structure and Doppler images.Conclusions: A miniature handheld OCT imager that can be used for intraoperative evaluation of microvascular anastomosis was successfully demonstrated.",
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