Mepacrine decreases lung leak in rats given interleukin-1 intratracheally

Young M. Lee, Brooks M. Hybertson, Lance S. Terada, Alexander J. Repine, Hyun G. Cho, John E. Repine

Research output: Contribution to journalArticle

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Abstract

We hypothesized that phospholipase A2 (PLA2) metabolites contribute to the acute, neutrophil-dependent, edematous lung leak that develops after administration of interleukin-1 (IL-1) intratracheally to rats and tested this premise by using mepacrine to inhibit PLA2 activity in vivo. We found that lung PLA2 activity, lung lavage phospholipid content, lung leak index, lung weight gain, and lung lavage protein concentrations were increased in rats given IL-1 intratracheally compared with sham-treated control rats. By comparison, lungs of mecaprine and IL-1-treated rats had decreased PLA2 activity, lavage phospholipid content, leak, weight gain, and lavage protein increases compared with rats given IL-1 intratracheally. Mepacrine treatment also decreased lung neutrophil accumulation, but not lung lavage cytokine- induced neutrophil chemoattractant (CINC) levels, in rats given IL-1 intratracheally. In parallel experiments, mepacrine treatment reduced the adhesion of human neutrophils to IL-1-treated human umbilical vein endothelial cells in vitro. Our results indicate that PLA2 activity participates in the lung neutrophil retention and pulmonary vascular leak that develops in rats given IL-1 intratracheally.

Original languageEnglish (US)
Pages (from-to)1624-1628
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume155
Issue number5
StatePublished - 1997

Fingerprint

Quinacrine
Interleukin-1
Phospholipases A2
Lung
Neutrophils
Bronchoalveolar Lavage
Therapeutic Irrigation
Weight Gain
Phospholipids
Chemotactic Factors
Human Umbilical Vein Endothelial Cells
Blood Vessels
Proteins
Cytokines
Therapeutics

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Lee, Y. M., Hybertson, B. M., Terada, L. S., Repine, A. J., Cho, H. G., & Repine, J. E. (1997). Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. American Journal of Respiratory and Critical Care Medicine, 155(5), 1624-1628.

Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. / Lee, Young M.; Hybertson, Brooks M.; Terada, Lance S.; Repine, Alexander J.; Cho, Hyun G.; Repine, John E.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 155, No. 5, 1997, p. 1624-1628.

Research output: Contribution to journalArticle

Lee, YM, Hybertson, BM, Terada, LS, Repine, AJ, Cho, HG & Repine, JE 1997, 'Mepacrine decreases lung leak in rats given interleukin-1 intratracheally', American Journal of Respiratory and Critical Care Medicine, vol. 155, no. 5, pp. 1624-1628.
Lee, Young M. ; Hybertson, Brooks M. ; Terada, Lance S. ; Repine, Alexander J. ; Cho, Hyun G. ; Repine, John E. / Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. In: American Journal of Respiratory and Critical Care Medicine. 1997 ; Vol. 155, No. 5. pp. 1624-1628.
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