Mesostriatal projections in BALB/c and CBA mice

A quantitative retrograde neuroanatomical tracing study

L. A. Mattiace, M. D. Baring, K. F. Manaye, G. A. Mihailoff, D. C. German

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A major portion of the midbrain dopamine (DA)-containing neurons project to the striatum and make up the mesostriatal DA system. The purpose of the present experiment was to map the location and quantify the density of mesostriatal neurons within two inbred mouse strains (BALB/c and CBA) known to possess different numbers of midbrain DA neurons. Computer-assisted reconstructions were made of both the wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) striatal injection site and the retrogradely labeled midbrain cells. There was no strain differences in the major source or topographical pattern of innervation of the striatum from the midbrain cellular regions. Even following small striatal injections, the labeled midbrain cells were found throughout most of the rostrocaudal extent of the midbrain DA nuclei; some labeled cells were found within the substantia nigra, the ventral tegmental area and the adjacent retrorubral field. Although the BALB/c strain has 20-25% more midbrain DA neurons than the CBA, given comparable striatal injection volumes, there was no significant difference in the number of HRP-filled mesostriatal neurons between the two mouse strains. These data suggest that the mesostriatal neurons give rise to comparable axonal branching within the striatum in the two mouse strains.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalBrain Research Bulletin
Volume23
Issue number1-2
DOIs
StatePublished - 1989

Fingerprint

Inbred CBA Mouse
Mesencephalon
Corpus Striatum
Dopaminergic Neurons
Neurons
Injections
Dopamine
Ventral Tegmental Area
Wheat Germ Agglutinins
Inbred Strains Mice
Substantia Nigra
Horseradish Peroxidase

Keywords

  • Horseradish peroxidase
  • Mesostriatal dopaminergic system
  • Mouse strains
  • Striatum
  • Substantia nigra Ventral tegmental area

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mesostriatal projections in BALB/c and CBA mice : A quantitative retrograde neuroanatomical tracing study. / Mattiace, L. A.; Baring, M. D.; Manaye, K. F.; Mihailoff, G. A.; German, D. C.

In: Brain Research Bulletin, Vol. 23, No. 1-2, 1989, p. 61-68.

Research output: Contribution to journalArticle

Mattiace, L. A. ; Baring, M. D. ; Manaye, K. F. ; Mihailoff, G. A. ; German, D. C. / Mesostriatal projections in BALB/c and CBA mice : A quantitative retrograde neuroanatomical tracing study. In: Brain Research Bulletin. 1989 ; Vol. 23, No. 1-2. pp. 61-68.
@article{3a9e776a1c4345d1ade7af3d60cc08dc,
title = "Mesostriatal projections in BALB/c and CBA mice: A quantitative retrograde neuroanatomical tracing study",
abstract = "A major portion of the midbrain dopamine (DA)-containing neurons project to the striatum and make up the mesostriatal DA system. The purpose of the present experiment was to map the location and quantify the density of mesostriatal neurons within two inbred mouse strains (BALB/c and CBA) known to possess different numbers of midbrain DA neurons. Computer-assisted reconstructions were made of both the wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) striatal injection site and the retrogradely labeled midbrain cells. There was no strain differences in the major source or topographical pattern of innervation of the striatum from the midbrain cellular regions. Even following small striatal injections, the labeled midbrain cells were found throughout most of the rostrocaudal extent of the midbrain DA nuclei; some labeled cells were found within the substantia nigra, the ventral tegmental area and the adjacent retrorubral field. Although the BALB/c strain has 20-25{\%} more midbrain DA neurons than the CBA, given comparable striatal injection volumes, there was no significant difference in the number of HRP-filled mesostriatal neurons between the two mouse strains. These data suggest that the mesostriatal neurons give rise to comparable axonal branching within the striatum in the two mouse strains.",
keywords = "Horseradish peroxidase, Mesostriatal dopaminergic system, Mouse strains, Striatum, Substantia nigra Ventral tegmental area",
author = "Mattiace, {L. A.} and Baring, {M. D.} and Manaye, {K. F.} and Mihailoff, {G. A.} and German, {D. C.}",
year = "1989",
doi = "10.1016/0361-9230(89)90164-0",
language = "English (US)",
volume = "23",
pages = "61--68",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "1-2",

}

TY - JOUR

T1 - Mesostriatal projections in BALB/c and CBA mice

T2 - A quantitative retrograde neuroanatomical tracing study

AU - Mattiace, L. A.

AU - Baring, M. D.

AU - Manaye, K. F.

AU - Mihailoff, G. A.

AU - German, D. C.

PY - 1989

Y1 - 1989

N2 - A major portion of the midbrain dopamine (DA)-containing neurons project to the striatum and make up the mesostriatal DA system. The purpose of the present experiment was to map the location and quantify the density of mesostriatal neurons within two inbred mouse strains (BALB/c and CBA) known to possess different numbers of midbrain DA neurons. Computer-assisted reconstructions were made of both the wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) striatal injection site and the retrogradely labeled midbrain cells. There was no strain differences in the major source or topographical pattern of innervation of the striatum from the midbrain cellular regions. Even following small striatal injections, the labeled midbrain cells were found throughout most of the rostrocaudal extent of the midbrain DA nuclei; some labeled cells were found within the substantia nigra, the ventral tegmental area and the adjacent retrorubral field. Although the BALB/c strain has 20-25% more midbrain DA neurons than the CBA, given comparable striatal injection volumes, there was no significant difference in the number of HRP-filled mesostriatal neurons between the two mouse strains. These data suggest that the mesostriatal neurons give rise to comparable axonal branching within the striatum in the two mouse strains.

AB - A major portion of the midbrain dopamine (DA)-containing neurons project to the striatum and make up the mesostriatal DA system. The purpose of the present experiment was to map the location and quantify the density of mesostriatal neurons within two inbred mouse strains (BALB/c and CBA) known to possess different numbers of midbrain DA neurons. Computer-assisted reconstructions were made of both the wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) striatal injection site and the retrogradely labeled midbrain cells. There was no strain differences in the major source or topographical pattern of innervation of the striatum from the midbrain cellular regions. Even following small striatal injections, the labeled midbrain cells were found throughout most of the rostrocaudal extent of the midbrain DA nuclei; some labeled cells were found within the substantia nigra, the ventral tegmental area and the adjacent retrorubral field. Although the BALB/c strain has 20-25% more midbrain DA neurons than the CBA, given comparable striatal injection volumes, there was no significant difference in the number of HRP-filled mesostriatal neurons between the two mouse strains. These data suggest that the mesostriatal neurons give rise to comparable axonal branching within the striatum in the two mouse strains.

KW - Horseradish peroxidase

KW - Mesostriatal dopaminergic system

KW - Mouse strains

KW - Striatum

KW - Substantia nigra Ventral tegmental area

UR - http://www.scopus.com/inward/record.url?scp=0024415125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024415125&partnerID=8YFLogxK

U2 - 10.1016/0361-9230(89)90164-0

DO - 10.1016/0361-9230(89)90164-0

M3 - Article

VL - 23

SP - 61

EP - 68

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 1-2

ER -