MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Xicheng Mao; Yafang Hu; Chao Liang; Changde Lu

doi:10.1007/s00284-001-0046-0

MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Xicheng Mao, Yafang Hu, Chao Liang, Changde Lu

Internal Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

Original language	English (US)
Pages (from-to)	37-40
Number of pages	4
Journal	Current Microbiology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1007/s00284-001-0046-0
State	Published - Jul 1 2002

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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AU - Mao, Xicheng

AU - Hu, Yafang

AU - Liang, Chao

AU - Lu, Changde

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