MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Xicheng Mao, Yafang Hu, Chao Liang, Changde Lu

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

Original languageEnglish (US)
Pages (from-to)37-40
Number of pages4
JournalCurrent Microbiology
Volume45
Issue number1
DOIs
StatePublished - Jul 1 2002

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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