MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Xicheng Mao; Yafang Hu; Chao Liang; Changde Lu

doi:10.1007/s00284-001-0046-0

MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Xicheng Mao, Yafang Hu, Chao Liang, Changde Lu

Internal Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

Original language	English (US)
Pages (from-to)	37-40
Number of pages	4
Journal	Current Microbiology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1007/s00284-001-0046-0
State	Published - Jul 1 2002

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

Access to Document

10.1007/s00284-001-0046-0

Cite this

@article{d0b2c0f92cf24cbd847fe34ac49f8670,

title = "MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain",

abstract = "The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.",

author = "Xicheng Mao and Yafang Hu and Chao Liang and Changde Lu",

year = "2002",

month = jul,

day = "1",

doi = "10.1007/s00284-001-0046-0",

language = "English (US)",

volume = "45",

pages = "37--40",

journal = "Current Microbiology",

issn = "0343-8651",

publisher = "Springer New York",

number = "1",

}

TY - JOUR

T1 - MET3 promoter

T2 - A tightly regulated promoter and its application in construction of conditional lethal strain

AU - Mao, Xicheng

AU - Hu, Yafang

AU - Liang, Chao

AU - Lu, Changde

PY - 2002/7/1

Y1 - 2002/7/1

N2 - The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

AB - The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

UR - http://www.scopus.com/inward/record.url?scp=0036636385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036636385&partnerID=8YFLogxK

U2 - 10.1007/s00284-001-0046-0

DO - 10.1007/s00284-001-0046-0

M3 - Article

C2 - 12029525

AN - SCOPUS:0036636385

VL - 45

SP - 37

EP - 40

JO - Current Microbiology

JF - Current Microbiology

SN - 0343-8651

IS - 1

ER -

MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this