Abstract
The β-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Δcln2Δpho85Δ) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-40 |
| Number of pages | 4 |
| Journal | Current Microbiology |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 2002 |
ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
Fingerprint Dive into the research topics of 'MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain'. Together they form a unique fingerprint.
Cite this
Mao, X., Hu, Y., Liang, C., & Lu, C. (2002). MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain. Current Microbiology, 45(1), 37-40. https://doi.org/10.1007/s00284-001-0046-0