Metabolic activity of brown, “beige,” and white adipose tissues in response to chronic adrenergic stimulation in male mice

Sébastien M. Labbé, Alexandre Caron, Kanta Chechi, Mathieu Laplante, Roger Lecomte, Denis Richard

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Classical brown adipocytes such as those found in interscapular brown adipose tissue (iBAT) represent energy-burning cells, which have been postulated to play a pivotal role in energy metabolism. Brown adipocytes can also be found in white adipose tissue (WAT) depots [e.g., inguinal WAT (iWAT)] following adrenergic stimulation, and they have been referred to as “beige” adipocytes. Whether the presence of these adipocytes, which gives iWAT a beige appearance, can confer a white depot with some thermogenic activity remains to be seen. In consequence, we designed the present study to investigate the metabolic activity of iBAT, iWAT, and epididymal white depots in mice. Mice were either 1) kept at thermoneutrality (30°C), 2) kept at 30°C and treated daily for 14 days with an adrenergic agonist [CL-316,243 (CL)], or 3) housed at 10°C for 14 days. Metabolic activity was assessed using positron emission tomography imaging with fluoro- [ 18 F]deoxyglucose (glucose uptake), fluoro-[ 18 F]thiaheptadecanoic acid (fatty acid uptake), and [ 11 C]acetate (oxidative activity). In each group, substrate uptakes and oxidative activity were measured in anesthetized mice in response to acute CL. Our results revealed iBAT as a major site of metabolic activity, which exhibited enhanced glucose and nonesterified fatty acid uptakes and oxidative activity in response to chronic cold and CL. On the other hand, beige adipose tissue failed to exhibit appreciable increase in oxidative activity in response to chronic cold and CL. Altogether, our results suggest that the contribution of beige fat to acute-CL-induced metabolic activity is low compared with that of iBAT, even after sustained adrenergic stimulation.

Original languageEnglish (US)
Pages (from-to)E260-E268
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume311
Issue number1
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Fingerprint

White Adipose Tissue
Brown Adipose Tissue
Adrenergic Agents
Groin
Brown Adipocytes
Glucose
Adrenergic Agonists
Deoxyglucose
Nonesterified Fatty Acids
Adipocytes
Positron-Emission Tomography
Energy Metabolism
Acetates
Fatty Acids
Acids
Beige Adipose Tissue

Keywords

  • Beige adipose tissue
  • Brown adipose tissue
  • Energy metabolism
  • Oxidative metabolism
  • Positron emission tomography

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Metabolic activity of brown, “beige,” and white adipose tissues in response to chronic adrenergic stimulation in male mice. / Labbé, Sébastien M.; Caron, Alexandre; Chechi, Kanta; Laplante, Mathieu; Lecomte, Roger; Richard, Denis.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 311, No. 1, 01.07.2016, p. E260-E268.

Research output: Contribution to journalArticle

Labbé, Sébastien M. ; Caron, Alexandre ; Chechi, Kanta ; Laplante, Mathieu ; Lecomte, Roger ; Richard, Denis. / Metabolic activity of brown, “beige,” and white adipose tissues in response to chronic adrenergic stimulation in male mice. In: American Journal of Physiology - Endocrinology and Metabolism. 2016 ; Vol. 311, No. 1. pp. E260-E268.
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