Metabolic characterization of hematopoietic stem cells

Fatih Kocabas; Junke Zheng; Chengcheng Zhang; Hesham A Sadek

doi:10.1007/978-1-4939-1133-2_10

Metabolic characterization of hematopoietic stem cells

Fatih Kocabas, Junke Zheng, Chengcheng Zhang, Hesham A Sadek

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10 Scopus citations

Abstract

An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.

Original language	English (US)
Pages (from-to)	155-164
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1185
DOIs	https://doi.org/10.1007/978-1-4939-1133-2_10
State	Published - 2014

ASJC Scopus subject areas

General Medicine

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10.1007/978-1-4939-1133-2_10

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author = "Fatih Kocabas and Junke Zheng and Chengcheng Zhang and Sadek, {Hesham A}",

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AU - Kocabas, Fatih

AU - Zheng, Junke

AU - Zhang, Chengcheng

AU - Sadek, Hesham A

PY - 2014

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AB - An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.

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JO - Methods in molecular biology (Clifton, N.J.)

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Metabolic characterization of hematopoietic stem cells

Abstract

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