Metabolic characterization of hematopoietic stem cells

Fatih Kocabas, Junke Zheng, Chengcheng Zhang, Hesham A Sadek

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume1185
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Medicine(all)

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