TY - JOUR
T1 - Metabolic effects of dietary stearic acid in mice
T2 - changes in the fatty acid composition of triglycerides and phospholipids in various tissues
AU - Bonanome, Andrea
AU - Bennett, Michael
AU - Grundy, Scott M
N1 - Funding Information:
This work was supported in part by the United States Veterans Administration, by Grant (HL-29252, l-RR0053, CA-433 11) of the National Institutes of Health, by the Southwestern Medical Foundation, by Mead Johnson and Company (Evansville, IN) and by the Moss Heart Foundation of Dallas, TX. The fat for the high stearic diet was provided by Mr. D.A. Leo. The authors are grateful to Julia Kope formerly of WL Clayton Research Center, Richardson, TX, Marjorie Whelan, Ray Wheatley for excellent technical help and to Beverley V. Adams for statistical analysis using the CLINFO Program.
PY - 1992/6
Y1 - 1992/6
N2 - The fatty acid patterns of triglycerides and phospholipids extracted from adipose tissue, liver, heart, kidney, spleen, and lung of 3 groups of C57BL/6 mice were determined after feeding diets rich in palmitic acid (16:0) (high palmitic: 16:0 = 45.1% of total fatty acids), stearic acid (18:0) (high stearic: 18:0 = 42.9% of total fatty acids) and oleic acid (18:1) (high oleic: 18:1 = 79.7% of total fatty acids) for 9 months. Triglyceride content of adipose, liver, heart, kidney, lung and spleen tissues was significantly enriched in palmitic acid in mice fed the high palmitic diet (range among all tissues: 19.9% ± 0.2% to 29.0% ± 1.9% of total fatty acids) and in oleic acid in mice fed the high oleic diet (range 56.0% ± 1.9% to 71.6% ± 1.2%). The stearic acid content of organ triglycerides in mice fed the high stearic diet ranged from 3.7% ± 0.3% to 10.8% ± 1.2%; however, the content of oleic acid on this diet (range; 57.0% ± 1.8% to 71.4% ± 1.7%) was similar to the one observed in mice fed the high oleic diet. In all organs, phospholipids had a significantly higher percentage of stearic acid (range: 23.5% ± 0.9% to 51.5% ± 6.6%) than triglycerides, regardless of diet. To evaluate the production of oleate from stearate and palmitate, 2 groups of mice were fed the high palmitic and the high stearic diets for 1 week and then injected intravenously with [1-14C]palmitate and [1-14C]stearate and the amount of labelled oleate in liver triglycerides was measured. In liver triglycerides of mice injected with [1-14C]stearic acid, more than 80% of 14C label recovered was bound to oleic acid, while in mice injected with radioactive palmitate, the maximum recovery of label bound to oleate was 47.0% ± 6.9%. These data suggest that stearic acid is rapidly converted into oleic acid and could help explain why dietary stearic acid does not raise plasma cholesterol.
AB - The fatty acid patterns of triglycerides and phospholipids extracted from adipose tissue, liver, heart, kidney, spleen, and lung of 3 groups of C57BL/6 mice were determined after feeding diets rich in palmitic acid (16:0) (high palmitic: 16:0 = 45.1% of total fatty acids), stearic acid (18:0) (high stearic: 18:0 = 42.9% of total fatty acids) and oleic acid (18:1) (high oleic: 18:1 = 79.7% of total fatty acids) for 9 months. Triglyceride content of adipose, liver, heart, kidney, lung and spleen tissues was significantly enriched in palmitic acid in mice fed the high palmitic diet (range among all tissues: 19.9% ± 0.2% to 29.0% ± 1.9% of total fatty acids) and in oleic acid in mice fed the high oleic diet (range 56.0% ± 1.9% to 71.6% ± 1.2%). The stearic acid content of organ triglycerides in mice fed the high stearic diet ranged from 3.7% ± 0.3% to 10.8% ± 1.2%; however, the content of oleic acid on this diet (range; 57.0% ± 1.8% to 71.4% ± 1.7%) was similar to the one observed in mice fed the high oleic diet. In all organs, phospholipids had a significantly higher percentage of stearic acid (range: 23.5% ± 0.9% to 51.5% ± 6.6%) than triglycerides, regardless of diet. To evaluate the production of oleate from stearate and palmitate, 2 groups of mice were fed the high palmitic and the high stearic diets for 1 week and then injected intravenously with [1-14C]palmitate and [1-14C]stearate and the amount of labelled oleate in liver triglycerides was measured. In liver triglycerides of mice injected with [1-14C]stearic acid, more than 80% of 14C label recovered was bound to oleic acid, while in mice injected with radioactive palmitate, the maximum recovery of label bound to oleate was 47.0% ± 6.9%. These data suggest that stearic acid is rapidly converted into oleic acid and could help explain why dietary stearic acid does not raise plasma cholesterol.
KW - Oleic acid
KW - Palmitic acid
KW - Phospholipids
KW - Stearic acid
KW - Triglycerides
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U2 - 10.1016/0021-9150(92)90236-A
DO - 10.1016/0021-9150(92)90236-A
M3 - Article
C2 - 1632865
AN - SCOPUS:0026654608
SN - 0021-9150
VL - 94
SP - 119
EP - 127
JO - Atherosclerosis
JF - Atherosclerosis
IS - 2-3
ER -