Metabolic labeling of glycoconjugates with photocrosslinking sugars

Seok Ho Yu, Michelle R. Bond, Chad M. Whitman, Jennifer J. Kohler

Research output: Book/ReportBook

12 Scopus citations

Abstract

Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages22
Volume478
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Enzymology
No.C
Volume478
ISSN (Print)00766879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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