Metabolic labeling of glycoconjugates with photocrosslinking sugars

Seok Ho Yu, Michelle R. Bond, Chad M. Whitman, Jennifer J. Kohler

Research output: Book/ReportBook

11 Citations (Scopus)

Abstract

Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages22
Volume478
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Enzymology
No.C
Volume478
ISSN (Print)00766879

Fingerprint

Glycoconjugates
Sugars
Labeling
Polysaccharides
Immunology
Metabolic Engineering
Biological Phenomena
Covalent bonds
Allergy and Immunology
Oligosaccharides
Carcinogenesis
Carbohydrates
Irradiation
Molecules
Infection
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Yu, S. H., Bond, M. R., Whitman, C. M., & Kohler, J. J. (2010). Metabolic labeling of glycoconjugates with photocrosslinking sugars. (C ed.) (Methods in Enzymology; Vol. 478, No. C). Unknown Publisher. https://doi.org/10.1016/S0076-6879(10)78026-5

Metabolic labeling of glycoconjugates with photocrosslinking sugars. / Yu, Seok Ho; Bond, Michelle R.; Whitman, Chad M.; Kohler, Jennifer J.

C ed. Unknown Publisher, 2010. 22 p. (Methods in Enzymology; Vol. 478, No. C).

Research output: Book/ReportBook

Yu, SH, Bond, MR, Whitman, CM & Kohler, JJ 2010, Metabolic labeling of glycoconjugates with photocrosslinking sugars. Methods in Enzymology, no. C, vol. 478, vol. 478, C edn, Unknown Publisher. https://doi.org/10.1016/S0076-6879(10)78026-5
Yu SH, Bond MR, Whitman CM, Kohler JJ. Metabolic labeling of glycoconjugates with photocrosslinking sugars. C ed. Unknown Publisher, 2010. 22 p. (Methods in Enzymology; C). https://doi.org/10.1016/S0076-6879(10)78026-5
Yu, Seok Ho ; Bond, Michelle R. ; Whitman, Chad M. ; Kohler, Jennifer J. / Metabolic labeling of glycoconjugates with photocrosslinking sugars. C ed. Unknown Publisher, 2010. 22 p. (Methods in Enzymology; C).
@book{1229076b6515446a9eeda3603b117753,
title = "Metabolic labeling of glycoconjugates with photocrosslinking sugars",
abstract = "Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.",
author = "Yu, {Seok Ho} and Bond, {Michelle R.} and Whitman, {Chad M.} and Kohler, {Jennifer J.}",
year = "2010",
doi = "10.1016/S0076-6879(10)78026-5",
language = "English (US)",
volume = "478",
series = "Methods in Enzymology",
publisher = "Unknown Publisher",
number = "C",
edition = "C",

}

TY - BOOK

T1 - Metabolic labeling of glycoconjugates with photocrosslinking sugars

AU - Yu, Seok Ho

AU - Bond, Michelle R.

AU - Whitman, Chad M.

AU - Kohler, Jennifer J.

PY - 2010

Y1 - 2010

N2 - Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.

AB - Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.

UR - http://www.scopus.com/inward/record.url?scp=77956293949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956293949&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(10)78026-5

DO - 10.1016/S0076-6879(10)78026-5

M3 - Book

C2 - 20816498

AN - SCOPUS:77956293949

VL - 478

T3 - Methods in Enzymology

BT - Metabolic labeling of glycoconjugates with photocrosslinking sugars

PB - Unknown Publisher

ER -