Metabolic profiling regarding pathogenesis of idiopathic pulmonary fibrosis

Yun Pyo Kang, Sae Bom Lee, Ji Min Lee, Hyung Min Kim, Ji Yeon Hong, Won Jun Lee, Chang Woo Choi, Hwa Kyun Shin, Do Jin Kim, Eun Suk Koh, Choon Sik Park, Sung Won Kwon, Sung Woo Park

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Idiopathic pulmonary fibrosis (IPF) is a progressive, eventually fatal disease characterized by fibrosis of the lung parenchyma and loss of lung function. IPF is believed to be caused by repetitive alveolar epithelial cell injury and dysregulated repair process including uncontrolled proliferation of lung (myo) fibroblasts and excessive deposition of extracellular matrix proteins in the interstitial space; however, the pathogenic pathways involved in IPF have not been fully elucidated. In this study, we attempted to characterize metabolic changes of lung tissues involved in the pathogenesis of IPF using gas chromatography-mass spectrometry-based metabolic profiling. Partial least-squares discriminant analysis (PLS-DA) model generated from metabolite data was able to discriminate between the control subjects and IPF patients (R2X = 0.37, R2Y = 0.613 and Q2 (cumulative) = 0.54, receiver operator characteristic AUC > 0.9). We discovered 25 metabolite signatures of IPF using both univariate and multivariate statistical analyses (FDR t; 0.05 and VIP score of PLS-DA > 1). These metabolite signatures indicated alteration in metabolic pathways: adenosine triphosphate degradation pathway, glycolysis pathway, glutathione biosynthesis pathway, and ornithine aminotransferase pathway. The results could provide additional insight into understanding the disease and potential for developing biomarkers.

Original languageEnglish (US)
Pages (from-to)1717-1724
Number of pages8
JournalJournal of Proteome Research
Volume15
Issue number5
DOIs
StatePublished - May 6 2016

Keywords

  • gas chromatography-mass spectrometry (GC-MS)
  • idiopathic pulmonary fibrosis
  • lung tissue
  • metabolic profiling

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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  • Cite this

    Kang, Y. P., Lee, S. B., Lee, J. M., Kim, H. M., Hong, J. Y., Lee, W. J., Choi, C. W., Shin, H. K., Kim, D. J., Koh, E. S., Park, C. S., Kwon, S. W., & Park, S. W. (2016). Metabolic profiling regarding pathogenesis of idiopathic pulmonary fibrosis. Journal of Proteome Research, 15(5), 1717-1724. https://doi.org/10.1021/acs.jproteome.6b00156