Metabolism of androstenedione by guinea-pig peritoneal macrophages: synthesis of testosterone and 5α-reduced metabolites

Leon Milewich, Grace T. Chen, Clifford Lyons, Thomas F. Tucker, Jonathan W. Uhr, Paul C. Macdonald

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Androstenedione metabolizing enzymes present in guinea-pig peritoneal macrophages were investigated using tritium-labeled androstenedionc as the substrate. We found that the metabolites of [3H]-androstenedione produced by these macrophages were testosterone. 5α-androstane-3,17-dione, isoandrosterone, androsterone, 5α-dihydrotestosterone, 5α-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol. The rates of metabolite formation remained linear as a function of time of incubation for approximately 30 min and with macrophage number up to 2 × 107 cells per ml. The formation of these metabolites is indicative that the following androstcnedione metabolizing enzymes are present in guinea-pig peritoneal macrophages: 5α-reductase, 3α-hydroxystcroid oxidoreductasc, 3β-hydroxysteroid oxidoreductase and 17β-hydroxystcroid oxidoreductasc. It is possible, therefore, that the macrophage, in vivo. may play a role in the metabolism of blood-borne androstcnedione to potent androgens. These hormones are important in the regulation of many biological processes, possibly including the activity of the macrophage itself.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalJournal of Steroid Biochemistry
Volume17
Issue number1
DOIs
StatePublished - 1982

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Androstenedione
Macrophages
Peritoneal Macrophages
Metabolites
Metabolism
Androstane-3,17-diol
Testosterone
Guinea Pigs
Oxidoreductases
Hydroxysteroids
Androsterone
Biological Phenomena
Tritium
Dihydrotestosterone
Enzymes
Androgens
Hormones
Blood
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Metabolism of androstenedione by guinea-pig peritoneal macrophages : synthesis of testosterone and 5α-reduced metabolites. / Milewich, Leon; Chen, Grace T.; Lyons, Clifford; Tucker, Thomas F.; Uhr, Jonathan W.; Macdonald, Paul C.

In: Journal of Steroid Biochemistry, Vol. 17, No. 1, 1982, p. 61-65.

Research output: Contribution to journalArticle

Milewich, Leon ; Chen, Grace T. ; Lyons, Clifford ; Tucker, Thomas F. ; Uhr, Jonathan W. ; Macdonald, Paul C. / Metabolism of androstenedione by guinea-pig peritoneal macrophages : synthesis of testosterone and 5α-reduced metabolites. In: Journal of Steroid Biochemistry. 1982 ; Vol. 17, No. 1. pp. 61-65.
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AB - Androstenedione metabolizing enzymes present in guinea-pig peritoneal macrophages were investigated using tritium-labeled androstenedionc as the substrate. We found that the metabolites of [3H]-androstenedione produced by these macrophages were testosterone. 5α-androstane-3,17-dione, isoandrosterone, androsterone, 5α-dihydrotestosterone, 5α-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol. The rates of metabolite formation remained linear as a function of time of incubation for approximately 30 min and with macrophage number up to 2 × 107 cells per ml. The formation of these metabolites is indicative that the following androstcnedione metabolizing enzymes are present in guinea-pig peritoneal macrophages: 5α-reductase, 3α-hydroxystcroid oxidoreductasc, 3β-hydroxysteroid oxidoreductase and 17β-hydroxystcroid oxidoreductasc. It is possible, therefore, that the macrophage, in vivo. may play a role in the metabolism of blood-borne androstcnedione to potent androgens. These hormones are important in the regulation of many biological processes, possibly including the activity of the macrophage itself.

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