TY - JOUR
T1 - Metabolism of androstenedione by guinea-pig peritoneal macrophages
T2 - synthesis of testosterone and 5α-reduced metabolites
AU - Milewich, Leon
AU - Chen, Grace T.
AU - Lyons, Clifford
AU - Tucker, Thomas F.
AU - Uhr, Jonathan W.
AU - Macdonald, Paul C.
N1 - Funding Information:
Supported, in part, by USPHS grants AGOO306. AI-11851 and AM27257. Address correspondence to: Dr Leon Milewich Department of Obstetrics and Gynecology. The University of Texas Southwestern Medical School. 5323 Harry Hines Boulevard. Dallas. Texas 75235. U.S.A.
PY - 1982/7
Y1 - 1982/7
N2 - Androstenedione metabolizing enzymes present in guinea-pig peritoneal macrophages were investigated using tritium-labeled androstenedionc as the substrate. We found that the metabolites of [3H]-androstenedione produced by these macrophages were testosterone. 5α-androstane-3,17-dione, isoandrosterone, androsterone, 5α-dihydrotestosterone, 5α-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol. The rates of metabolite formation remained linear as a function of time of incubation for approximately 30 min and with macrophage number up to 2 × 107 cells per ml. The formation of these metabolites is indicative that the following androstcnedione metabolizing enzymes are present in guinea-pig peritoneal macrophages: 5α-reductase, 3α-hydroxystcroid oxidoreductasc, 3β-hydroxysteroid oxidoreductase and 17β-hydroxystcroid oxidoreductasc. It is possible, therefore, that the macrophage, in vivo. may play a role in the metabolism of blood-borne androstcnedione to potent androgens. These hormones are important in the regulation of many biological processes, possibly including the activity of the macrophage itself.
AB - Androstenedione metabolizing enzymes present in guinea-pig peritoneal macrophages were investigated using tritium-labeled androstenedionc as the substrate. We found that the metabolites of [3H]-androstenedione produced by these macrophages were testosterone. 5α-androstane-3,17-dione, isoandrosterone, androsterone, 5α-dihydrotestosterone, 5α-androstane-3α,17β-diol and 5α-androstane-3β,17β-diol. The rates of metabolite formation remained linear as a function of time of incubation for approximately 30 min and with macrophage number up to 2 × 107 cells per ml. The formation of these metabolites is indicative that the following androstcnedione metabolizing enzymes are present in guinea-pig peritoneal macrophages: 5α-reductase, 3α-hydroxystcroid oxidoreductasc, 3β-hydroxysteroid oxidoreductase and 17β-hydroxystcroid oxidoreductasc. It is possible, therefore, that the macrophage, in vivo. may play a role in the metabolism of blood-borne androstcnedione to potent androgens. These hormones are important in the regulation of many biological processes, possibly including the activity of the macrophage itself.
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U2 - 10.1016/0022-4731(82)90592-1
DO - 10.1016/0022-4731(82)90592-1
M3 - Article
C2 - 7109593
AN - SCOPUS:0020399615
SN - 0022-4731
VL - 17
SP - 61
EP - 65
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 1
ER -