Method for inducing experimental pneumococcal meningitis in outbred mice

Damiana Chiavolini, Sergio Tripodi, Riccardo Parigi, Marco R. Oggioni, Elisabetta Blasi, Marcella Cintorino, Gianni Pozzi, Susanna Ricci

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. Results: In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50% of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. Conclusions: The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.

Original languageEnglish (US)
Article number36
JournalBMC Microbiology
Volume4
DOIs
StatePublished - Sep 22 2004

Fingerprint

Pneumococcal Meningitis
Streptococcus pneumoniae
Bacterial Meningitides
Meningitis
Subarachnoid Space
Lethal Dose 50
Infection
Stem Cells
Vaccines
Spleen
Animal Models
Anti-Bacterial Agents
Bacteria
Costs and Cost Analysis
Mortality
Brain
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Chiavolini, D., Tripodi, S., Parigi, R., Oggioni, M. R., Blasi, E., Cintorino, M., ... Ricci, S. (2004). Method for inducing experimental pneumococcal meningitis in outbred mice. BMC Microbiology, 4, [36]. https://doi.org/10.1186/1471-2180-4-36

Method for inducing experimental pneumococcal meningitis in outbred mice. / Chiavolini, Damiana; Tripodi, Sergio; Parigi, Riccardo; Oggioni, Marco R.; Blasi, Elisabetta; Cintorino, Marcella; Pozzi, Gianni; Ricci, Susanna.

In: BMC Microbiology, Vol. 4, 36, 22.09.2004.

Research output: Contribution to journalArticle

Chiavolini, D, Tripodi, S, Parigi, R, Oggioni, MR, Blasi, E, Cintorino, M, Pozzi, G & Ricci, S 2004, 'Method for inducing experimental pneumococcal meningitis in outbred mice', BMC Microbiology, vol. 4, 36. https://doi.org/10.1186/1471-2180-4-36
Chiavolini, Damiana ; Tripodi, Sergio ; Parigi, Riccardo ; Oggioni, Marco R. ; Blasi, Elisabetta ; Cintorino, Marcella ; Pozzi, Gianni ; Ricci, Susanna. / Method for inducing experimental pneumococcal meningitis in outbred mice. In: BMC Microbiology. 2004 ; Vol. 4.
@article{5bb675c5540e4f21bed855896acc4091,
title = "Method for inducing experimental pneumococcal meningitis in outbred mice",
abstract = "Background: Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. Results: In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50{\%} of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. Conclusions: The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.",
author = "Damiana Chiavolini and Sergio Tripodi and Riccardo Parigi and Oggioni, {Marco R.} and Elisabetta Blasi and Marcella Cintorino and Gianni Pozzi and Susanna Ricci",
year = "2004",
month = "9",
day = "22",
doi = "10.1186/1471-2180-4-36",
language = "English (US)",
volume = "4",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Method for inducing experimental pneumococcal meningitis in outbred mice

AU - Chiavolini, Damiana

AU - Tripodi, Sergio

AU - Parigi, Riccardo

AU - Oggioni, Marco R.

AU - Blasi, Elisabetta

AU - Cintorino, Marcella

AU - Pozzi, Gianni

AU - Ricci, Susanna

PY - 2004/9/22

Y1 - 2004/9/22

N2 - Background: Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. Results: In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50% of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. Conclusions: The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.

AB - Background: Streptococcus pneumoniae is the leading cause of bacterial meningitis. Pneumococcal meningitis is associated with the highest mortality among bacterial meningitis and it may also lead to neurological sequelae despite the use of antibiotic therapy. Experimental animal models of pneumococcal meningitis are important to study the pathogenesis of meningitis, the host immune response induced after infection, and the efficacy of novel drugs and vaccines. Results: In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50% of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. Conclusions: The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.

UR - http://www.scopus.com/inward/record.url?scp=8844232717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8844232717&partnerID=8YFLogxK

U2 - 10.1186/1471-2180-4-36

DO - 10.1186/1471-2180-4-36

M3 - Article

C2 - 15385055

AN - SCOPUS:8844232717

VL - 4

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

M1 - 36

ER -